公开(公告)号：EP1506217A4

公开(公告)日：2006-04-12

申请号：EP03731453

申请日：2003-05-29

申请人： JACKSON H M FOUND MILITARY MED

发明人： POLLARD HARVEY B

IPC分类号： C12N9/16 ,  C12Q1/68 ,  C07K1/00 ,  G01N33/68 ,  G01T1/29

CPC分类号： C12Q1/6813 ,  C12N9/16 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2600/158 ,  C12Q2563/101

摘要： Cystic fibrosis (CF) is the most common fatal autosomal recessive disease in the U.S. and is principally caused by the DF508 mutation in the CFTR gene. The principal site of morbidity and mortality for this disease is the lung. We have used genomic and proteomic methods to identify ubiquitin carboxy terminal hydrolase-1 (UCHL1) as a biomarker for cystic fibrosis. Both gene expression and cognate protein expression are massively upregulated in CF lung epithelial cells. We suggest that this gene can be useful in the assembly of a diagnostic or prognostic chip for CF, or as a target for therapeutic intervention.

公开(公告)号：EP0857221A4

公开(公告)日：2004-05-12

申请号：EP97927859

申请日：1997-06-02

申请人： POLYPROBE INC

发明人： NILSEN THOR W

IPC分类号： C12N15/09 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/682 ,  C12Q1/6841 ,  C12Q2563/107 ,  C12Q2525/313 ,  C12Q2525/151 ,  C12Q2563/101 ,  C12Q2525/113

摘要： A method for the preparation of optimally fluorescent oligonucleotides wherein fluorescent dye-conjugated nucleotide triphosphates are incorporated into a nucleic acid sequence in a defined repetitive manner which allows for the optimal specific fluorescence of the oligonucleotide. The oligonucleotides of the present invention are useful in the assay of a wide variety of nucleic acid sequences, specifically wherever highly fluorescent nucleic acid probes are desired.

公开(公告)号：EP1114178A1

公开(公告)日：2001-07-11

申请号：EP99930461.1

申请日：1999-06-18

申请人： INCYTE PHARMACEUTICALS, INC.

发明人： JU, Jingyue ,  KONRAD, Ken

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C12Q2563/101 ,  C12Q2535/101 ,  C12Q2525/186 ,  C12Q2565/518 ,  C12Q2525/204

摘要： Methods of enzymatic nucleic acid sequencing are provided in which solid-phase capturable chain terminators are employed. In the subject methods, sequencing fragments are generated, where the fragments comprise capturable chain terminators. The fragments are then captured on a solid phase and separated from the remaining components of the sequencing reaction. The fragments are then released from the solid phase, size separated and detected to yield sequencing data from which the sequence of the nucleic acid is determined.

公开(公告)号：EP0668929B1

公开(公告)日：1997-08-13

申请号：EP92911283.7

申请日：1992-06-05

申请人： AMERSHAM INTERNATIONAL plc

发明人： BLAIR, James, Ninian, Rowell 39 Old Church Road ,  DOWNES, Malcolm, John ,  PARRY, David, Alun Flat 2 ,  CROSS, Lisa ,  ROBINSON, Philip, Steven 98 Winslow Road Wingrave

IPC分类号： C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  C12Q2563/131 ,  C12Q2563/101 ,  C12Q2545/113

摘要： A method of amplifying and quantitating a specific nucleic acid sequence in a sample using at least one oligonucleotide primer, a supply of nucleotides and optionally a probe complementary to part of the sequence, one of these including a radionuclide. In the presence of a polymerase the reagents form an amplified product based on the specific nucleic acid sequence. The amplified sequence is allowed to bind to a solid material comprising a fluorescer which fluoresces in proximity to the radionuclide and fluorescence is observed as a measure of the specific nucleic acid sequence. The method can be applied to a range of well-known nucleic acid amplification techniques such as the Polymerase Chain Reaction. A variation on the above method allows use of the Ligase Chain Reaction technique.

公开(公告)号：EP0183800A1

公开(公告)日：1986-06-11

申请号：EP85902901.0

申请日：1985-05-29

申请人： RODLAND, Karin D.

发明人： RODLAND, Karin D.

IPC分类号： C07H21 ,  C12Q1

CPC分类号： C12Q1/6895 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q2563/131 ,  C12Q2563/101 ,  C12Q2525/113

摘要： Compositions et procédé d'investigation par hybridation pour détecter des acides nucléiques. Une molécule d'investigation par hybridation est construite de façon à contenir des thionucléotides et est associée par hybridation à une séquence nucléotide d'intérêt en l'absence de formamide, de DDT et de sulfate de dextran. Si un thionucléotide froid (32S) est incorporé à la molécule d'investigation, celle-ci est pourvue d'une étiquette détectable par radioactivité ou autrement.

公开(公告)号：EP3010929A4

公开(公告)日：2017-02-22

申请号：EP14812900

申请日：2014-06-16

申请人： UNIV COLUMBIA

发明人： BESTOR TIMOTHY H ,  JU JINGYUE ,  LI XIAOXU

IPC分类号： C07H19/167 ,  C07H1/00 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C07H19/167 ,  C07H1/00 ,  C07H21/04 ,  C12Q1/6874 ,  C12Q2523/125 ,  C12Q2535/101 ,  C12Q2563/107 ,  C12Q2563/101

公开(公告)号：EP3010929A1

公开(公告)日：2016-04-27

申请号：EP14812900.0

申请日：2014-06-16

申请人： The Trustees of Columbia University in the City of New York

发明人： BESTOR, Timothy H. ,  JU, Jingyue ,  LI, Xiaoxu

IPC分类号： C07H19/23 ,  C07H19/167 ,  C07H19/173

CPC分类号： C07H19/167 ,  C07H1/00 ,  C07H21/04 ,  C12Q1/6874 ,  C12Q2523/125 ,  C12Q2535/101 ,  C12Q2563/107 ,  C12Q2563/101

摘要： The present invention provides a compound having the adenosylmethionine structure, wherein the sulfur atom of the methionine moiety is modified with alkene or succinimide groups, wherein R1, R2 and R3 are independently H, alkyl, aryl, C(0)NH2, C(0)R', CN, N02, C (O)R', S(0),NHR'; wherein X is 0 or NR'; wherein R' is H, alkyl or aryl; and wherein n is an integer from 1 to 8, with the proviso that, when substituent is a propene derivative, and n is 1, at least one of R1, R2, or R3 is other than H.

公开(公告)号：EP1358357B1

公开(公告)日：2011-03-09

申请号：EP02716498.7

申请日：2002-01-11

申请人： Biomolex AS

发明人： HOVIG, Eivind ,  SKRETTING, Arne ,  NYGARD, Einar ,  KVINNSLAND, Yngve ,  BREISTOL, Knut ,  YOSHIOKA, Koki

IPC分类号： C12Q1/68 ,  G01N33/543 ,  G01T1/164

CPC分类号： C40B30/04 ,  C12Q1/6816 ,  C12Q1/6837 ,  G01N33/60 ,  G01N33/6842 ,  G01N33/6845 ,  C12Q2565/501 ,  C12Q2563/101 ,  C12Q2545/114

摘要： The present invention relates to a method and apparatus for simultaneous quantification of the amounts of one or more radioactive nuclides within arbitrary regions on a surface where these nuclides have been deposited, adsorbed or fixed. These radioactive nuclides serve as markers on compounds that typically have been incorporated into tissue sections or into larger biological molecules that by various mechanisms have been bound to chemical substances on this surface. The method is especially well suited for DNA microarray deductions through the use of nucleotides labelled with different beta-emitting radionuclides.

公开(公告)号：EP1041145B1

公开(公告)日：2006-10-18

申请号：EP00106939.2

申请日：2000-03-31

申请人： Taiyo Nippon Sanso Corporation

发明人： Kawai, Gota ,  Wada, Akira ,  Takaku, Hiroshi

IPC分类号： C12N15/11 ,  C12Q1/68

CPC分类号： C12N15/113 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/3517 ,  C12Q1/68 ,  Y10T436/24 ,  C12Q2565/633 ,  C12Q2563/101

公开(公告)号：EP0668929A1

公开(公告)日：1995-08-30

申请号：EP92911283.0

申请日：1992-06-05

申请人： AMERSHAM INTERNATIONAL plc

发明人： BLAIR, James, Ninian, Rowell 39 Old Church Road ,  DOWNES, Malcolm, John ,  PARRY, David, Alun Flat 2 ,  CROSS, Lisa ,  ROBINSON, Philip, Steven 98 Winslow Road Wingrave

IPC分类号： C12Q1

CPC分类号： C12Q1/6818 ,  C12Q1/6851 ,  C12Q2563/131 ,  C12Q2563/101 ,  C12Q2545/113

摘要： A method of amplifying and quantitating a specific nucleic acid sequence in a sample using at least one oligonucleotide primer, a supply of nucleotides and optionally a probe complementary to part of the sequence, one of these including a radionuclide. In the presence of a polymerase the reagents form an amplified product based on the specific nucleic acid sequence. The amplified sequence is allowed to bind to a solid material comprising a fluorescer which fluoresces in proximity to the radionuclide and fluorescence is observed as a measure of the specific nucleic acid sequence. The method can be applied to a range of well-known nucleic acid amplification techniques such as the Polymerase Chain Reaction. A variation on the above method allows use of the Ligase Chain Reaction technique.