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1.发明公开
公开(公告)号：EP3913068A1
公开(公告)日：2021-11-24
申请号：EP21181324.1
申请日：2016-10-11
发明人： LUO, Yuling , WU, Xingyong , PAN, Liuliu , WANG, Xiaoming , MA, Xiao-Jun , SU, Nan , CHEN, Steve
IPC分类号： C12Q1/682 , C12Q1/6841
摘要： The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.
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2.发明公开
公开(公告)号：EP3362462A1
公开(公告)日：2018-08-22
申请号：EP16856049.8
申请日：2016-10-11
发明人： LUO, Yuling , WU, Xingyong , PAN, Liuliu , WANG, Xiaoming , MA, Xiao-Jun , SU, Nan , CHEN, Steve
CPC分类号： C12Q1/6841 , C12Q1/682 , C12Q1/6827 , C12Q2565/102
摘要： The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample. The invention also provides samples, tissue slides, and kits relating to detection of nucleic acid variations, including single nucleotide variations, multi-nucleotide variations or splice sites, of a target nucleic acid.
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