公开(公告)号：EP2809784A1

公开(公告)日：2014-12-10

申请号：EP13742978.3

申请日：2013-01-22

申请人： Advanced Liquid Logic, Inc. ,  Leitner, Thomas

发明人： LEITNER, Thomas

IPC分类号： C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/703 ,  C12Q2600/112

摘要： The present invention relates to amplification oligonucleotides (primers) and detection probes for the detection of human immunodeficiency virus type-1 (HIV-1) in a test sample. The primers and probes may be used in an assay for the detection and/or quantitation of HIV-1 nucleic acid. In various embodiments, the primers and detection probes may be used for real-time reverse transcription polymerase chain reaction (RT-PCR) assays for detection of HIV-1 nucleic acid. The detection probes may, for example, be fluorescently-labeled TaqMan® probes. In one embodiment, amplification primers and probes may be specific for the pol region of the HIV-1 genome. In another embodiment, the amplification primers and probes may be specific for the env region of the HIV-1 genome. "Cocktails" of multiple amplification primers and/or probes may be used to achieve maximal coverage of different HIV-1 subtypes.