公开(公告)号：EP2408899A1

公开(公告)日：2012-01-25

申请号：EP10753788.8

申请日：2010-03-19

申请人： Agency for Science, Technology and Research

发明人： LI, Mo Huang ,  YING, Jackie Y. ,  WEI, Jiashen ,  WAI, Chye Cheong

IPC分类号： C12M1/12 ,  C12M3/06 ,  B01D69/00 ,  G01N33/48

CPC分类号： B01D67/0062 ,  B01D63/087 ,  B01D69/02 ,  B01D69/10 ,  B01D71/02 ,  B01D2325/028 ,  B01L3/5023 ,  B01L3/5635 ,  B01L2200/025

摘要： The present invention refers to a device for separating cells of a defined size from a sample and to a system comprising such devices and further components. Such devices can comprise different components, such as inlet module, intermediate module, outlet module and microsieve. The present invention also refers to methods of operating such devices and uses thereof.

公开(公告)号：EP2430180A1

公开(公告)日：2012-03-21

申请号：EP09844717.0

申请日：2009-05-11

申请人： Agency for Science, Technology and Research

发明人： LI, Mo Huang ,  YING, Y. , Jackie ,  WAI, Chye Cheong ,  BODE, Marcus

IPC分类号： C12Q1/68 ,  C12N15/11 ,  C12P19/34

CPC分类号： C12Q1/686 ,  C12N15/1096 ,  C12Q1/6811 ,  C12Q2527/107 ,  C12Q2525/204

摘要： The present invention relates to polymerase chain reaction (PCR)-based methods for the one-step synthesis of nucleic acid molecules, wherein the amplification primers used in said methods are designed such that they have two distinct melting temperatures in order to minimize the competition between polymerase cycling assembly (PCA) and polymerase chain reaction (PCR) amplification in the one-step nucleic acid synthesis and to maximize the emerging full-length amplification, as well as kits for use in such methods.

摘要翻译： 本发明涉及用于一步合成核酸分子的基于聚合酶链式反应（PCR）的方法，其中用于所述方法中的扩增引物被设计为使得它们具有两个不同的解链温度以最小化 聚合酶循环装配（PCA）和聚合酶链式反应（PCR）扩增在一步核酸合成中最大化新出现的全长扩增，以及用于这种方法的试剂盒。