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    NANOPROBE-BASED GENETIC TESTING
    4.
    发明公开
    NANOPROBE-BASED GENETIC TESTING 审中-公开
    基于纳米粒子的遗传检测

    公开(公告)号：EP2984183A1

    公开(公告)日：2016-02-17

    申请号：EP14783008.7

    申请日：2014-04-04

    申请人： Agency for Science, Technology and Research

    发明人： ZU, Yanbing ,  YING, Jackie Y.

    IPC分类号： C12Q1/68 ,  B82Y15/00 ,  C07H21/00

    摘要： The present application relates to methods of detecting a mutation in a target nucleic acid molecule. Two phosphorodiamidate morpholino oligomer probes that differ by at least one base are each covalently coupled to a nano article and hybridized to a target sequence. The melting temperature of the complexes between each of the two probes and the target nucleic acid are measured and compared to determine whether the sample contains a nucleic acid with the mutation. Further, the present invention relates to kits comprising a first and second conjugate as described herein and to the use of such kits for the detection of mutations in a target nucleic acid molecule or for assigning a genotype to a target nucleic acid molecule.

    摘要翻译： 本申请涉及检测靶核酸分子中的突变的方法。 两个不同于至少一个碱基的磷酰二胺吗啉代寡聚物探针各自共价偶联至纳米颗粒并与靶序列杂交。 测量两种探针中的每种探针与靶核酸之间的复合物的解链温度并进行比较以确定样品是否含有具有突变的核酸。 此外，本发明涉及包含如本文所述的第一和第二缀合物的试剂盒，并涉及此类试剂盒用于检测靶核酸分子中的突变或将基因型分配至靶核酸分子的用途。

    N-HETEROCYCLIC CARBENE CATALYZED SYNTHESIS OF N-PHENYLISOXAZOLIDIN-5-ONE DERIVATIVE AND SYNTHESIS OF ß-AMINO ACID ESTER DERIVATIVE
    8.
    发明公开
    N-HETEROCYCLIC CARBENE CATALYZED SYNTHESIS OF N-PHENYLISOXAZOLIDIN-5-ONE DERIVATIVE AND SYNTHESIS OF ß-AMINO ACID ESTER DERIVATIVE 审中-公开
    用n-PHENYLISOXAZOLIDIN -5-酮衍生物和合成？氨基酸酯衍生物性功能的N-杂环卡宾催化合成

    公开(公告)号：EP2139873A1

    公开(公告)日：2010-01-06

    申请号：EP08724343.2

    申请日：2008-03-14

    申请人： Agency for Science, Technology and Research

    发明人： ZHANG, Yugen ,  YING, Jackie Y. ,  SEAYAD, Jayasree ,  PATRA, Pranab, K.

    IPC分类号： C07D261/04 ,  C07C227/18 ,  C07C229/18

    CPC分类号： C07C227/18 ,  C07D261/04 ,  C07C229/18

    摘要： A process for preparing a N-phenylisoxazolidin-5-one derivative is provided. The process for preparing the N-phenylisoxazolidin-5-one derivative comprises reacting an α, β-unsaturated aldehyde with nitrosobenzene in the presence of a N-heterocyclic carbene (NHC) catalyst. A process for preparing an N-alkoxyphenyl protected β-amino acid ester derivative is further provided. The process for preparing the N-alkoxyphenyl protected β-amino acid ester derivative comprises reacting an α, β-unsaturated aldehyde with nitrosobenzene in the presence of a N-heterocyclic carbene (NHC) catalyst to form a N-phenylisoxazolidin-5-one derivative, and then treating the N-phenylisoxazolidin-5-one derivative with an alcohol in the presence of an acid catalyst. A process for preparing an N-alkoxyphenyl protected β-amino acid ester derivative, comprising treating a N-phenylisoxazolidin-5-one derivative with an alcohol in the presence of an acid catalyst, is also provided.