公开(公告)号：EP2617428A1

公开(公告)日：2013-07-24

申请号：EP13164423.9

申请日：2007-08-15

申请人： Agency for Science, Technology and Research

发明人： Lye, Elias ,  Lim, Sai Kiang

IPC分类号： A61K35/28 ,  C12N5/071

CPC分类号： A61K35/28 ,  C12N5/0018 ,  C12N5/0606 ,  C12N5/0611 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0668 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2506/02 ,  C12N2509/00

摘要： We disclose a method of preparing a conditioned cell culture medium, the method comprising the steps of: (a) culturing a mesenchymal stem cell (MSC), a descendent thereof or a cell line derived therefrom in a cell culture medium; and (b) optionally isolating the cell culture medium; in which the mesenchymal stem cell (MSC) is obtained by propagating a cell obtained by dispersing a embryonic stem (ES) cell colony, or a descendent thereof, in the absence of co-culture in a serum free medium comprising FGF2.

摘要翻译： 我们公开了一种制备条件细胞培养基的方法，所述方法包括以下步骤：（a）在细胞培养基中培养间充质干细胞（MSC），其后代或由其衍生的细胞系; 和（b）任选地分离细胞培养基; 其中间充质干细胞（MSC）是通过在不含共培养的情况下将包含FGF2的无血清培养基分散胚胎干（ES）细胞集落或其后代所获得的细胞获得的。