公开(公告)号：EP0974653A2

公开(公告)日：2000-01-26

申请号：EP99305469.1

申请日：1999-07-09

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Torigoe, Toshihiko ,  Sahara, Hiroeki ,  Suzuki, Manabu, c/o Ajinomoto Co., Inc. ,  Hamuro, Junji, c/o Ajinomoto Co., Inc.

IPC分类号： C12N15/12 ,  C07K14/47 ,  A61K38/17 ,  C07K7/06

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00

摘要： A tumor antigen gene is identified by screening a cDNA library derived from a gastric cancer cell line that can induce gastric cancer antigen specific cytotoxic T cell (CTL) by means of hybridization and PCR utilizing an amino acid sequence of peptide fragment of a known gastric cancer antigen protein, introducing a selected cDNA clone into a cell of gastric cancer cell line that cannot induce gastric cancer antigen specific CTL so that the clone should be expressed in the cell, and selecting a transgenic cell that has acquired the ability to induce CTL. According to the present invention, there are provided a protein capable of inducing immune response against human gastric cancer, DNA encoding the protein, as well as vaccine for treatment and prevention of human gastric cancer, and agent for treatment and prevention of human gastric cancer.

摘要翻译： 通过筛选来自胃癌细胞系的cDNA文库来鉴定肿瘤抗原基因，该cDNA文库可以通过使用已知胃癌的肽片段的氨基酸序列的杂交和PCR来诱导胃癌抗原特异性细胞毒性T细胞（CTL） 将选择的cDNA克隆引入到不能诱导胃癌抗原特异性CTL的胃癌细胞系的细胞中，使得克隆应在细胞中表达，并选择已经获得诱导CTL的能力的转基因细胞。 根据本发明，提供能够诱导针对人胃癌的免疫应答的蛋白质，编码该蛋白质的DNA，以及用于治疗和预防人胃癌的疫苗，以及用于治疗和预防人胃癌的药剂。

公开(公告)号：EP0974653B1

公开(公告)日：2005-09-14

申请号：EP99305469.1

申请日：1999-07-09

申请人： Ajinomoto Co., Inc. ,  Kikuchi, Kokichi

发明人： Kikuchi, Kokichi ,  Sato, Noriyuki ,  Torigoe, Toshihiko ,  Sahara, Hiroeki ,  Suzuki, Manabu, c/o Ajinomoto Co., Inc. ,  Hamuro, Junji, c/o Ajinomoto Co., Inc.

IPC分类号： C12N15/12 ,  C07K14/47 ,  A61K38/17 ,  C07K7/06

CPC分类号： C07K14/4748 ,  A61K38/00 ,  A61K39/00