公开(公告)号：EP1182261A1

公开(公告)日：2002-02-27

申请号：EP01120248.8

申请日：2001-08-23

申请人： Ajinomoto Co., Ltd.

发明人： Kobayashi, Masaki ,  Itoyama, Tsuyoshi ,  Mitani, Yukio ,  Usui, Naoki

IPC分类号： C12P13/08 ,  C12P13/10 ,  C12P13/24 ,  C12P13/04 ,  C07C229/26 ,  C07C229/28 ,  C07D233/64

CPC分类号： C12P13/10 ,  C12P13/08 ,  C12P13/24 ,  Y02P20/142

摘要： When a basic amino acid is produced by culturing a microorganism having an ability to produce the basic amino acid in a liquid medium under an aerobic condition to produce and accumulate the basic amino acid in the medium, pH of the medium is controlled to be 6.5-9.0 during the culture, and 7.2-9.0 at the end of the culture, and a culture period where 2 g/L or more of hydrogencarbonate ions and/or carbonate ions exist in the medium is secured during the culture by controlling pressure in a fermentation tank to be a positive pressure during the fermentation, or supplying carbon dioxide gas or a mixed gas containing carbon dioxide gas to the medium, so that the hydrogencarbonate ions and/or carbonate ions should serve as counter ions of cations mainly consisting of the basic amino acid.

摘要翻译： 当在需氧条件下培养具有在液体培养基中生产碱性氨基酸的能力的微生物产生碱性氨基酸以在培养基中产生和积累碱性氨基酸时，将培养基的pH控制在6.5- 9.0，在培养结束时为7.2-9.0，培养期间通过控制发酵中的压力确保培养中存在2g / L以上碳酸氢根离子和/或碳酸根离子的培养期 罐在发酵过程中为正压，或向介质供应二氧化碳气体或含二氧化碳气体的混合气体，以使碳酸氢根离子和/或碳酸根离子作为主要由碱性氨基 酸。