摘要:
Known diffusion chambers for measuring the pH value and absorption of aqueous substance samples take measurements with a relatively low resolution, large substance sample quantities, or in a non-simultaneous manner in the same sample. The diffusion chamber (01) according to the invention is equipped with a high-resolution pH optode (07) and a compact spectrophotometer (13), the respective glass fiber optics of which are integrated into the central cylinder (03) of the diffusion chamber (01) by means of special structural measures. In the process, the pH optode (07) is designed in the form of a syringe and is inserted into a sample slide (20), the cannula (11) being inserted into a diagonal groove (32) and guided through a sealing ring (22) on the sample slide (20) in a gas-tight manner. Thus, very small substance samples (05) (
摘要:
Known high-pressure-resistant specimen chambers have complex progressive metal seals. Non-high-pressure-resistant specimen chambers have complex clamps for the viewing windows. In the case of the high-pressure-resistant specimen chamber (01) according to the invention, viewing windows (07, 08) having plane-parallel raised areas (23, 25), which protrude into viewing openings (13, 14) of clamping elements (11, 12) in an accurately fitting manner, and high-pressure-resistant seals (09, 10) are provided. Thus, a compact, very well joined high-pressure-resistant specimen chamber results. The elements are composed of a plastomer that can be plastically deformed when high pressure is applied. In the production, flat disks (32, 34) composed of polycarbonate and sealing rings (31, 33) composed of polytetrafluoroethylene are inserted into the specimen chamber (01) and a pressure above the pressure at which plastic deformation starts is applied to the specimen chamber after the final assembly. The flat disks (32, 34) and the sealing rings (31, 33) thereby form the viewing windows (07, 08) and high-pressure-resistant seals (09, 10). The specimen chamber (01) can be used as a parameterizable flow-through chamber for the microscopic in situ observation of living organisms under an overpressure corresponding to the high pressure that is used, said overpressure corresponding to the depth of the sea, e.g., 500 m and more, of the habitat of the organisms.