DIFFUSIONSKAMMER ZUR ERMITTLUNG UNTERSCHIEDLICHER PARAMETER EINER WÄSSRIGEN SUBSTANZ
    3.
    发明公开
    DIFFUSIONSKAMMER ZUR ERMITTLUNG UNTERSCHIEDLICHER PARAMETER EINER WÄSSRIGEN SUBSTANZ 有权
    DIFFUSIONSKAMMER ZUR ERMITTLUNG UNTERSCHIEDLICHER PARAMETER EINERWÄSSRIGENSUBSTANZ

    公开(公告)号:EP3017290A2

    公开(公告)日:2016-05-11

    申请号:EP14789977.7

    申请日:2014-06-21

    IPC分类号: G01N21/01 G01N21/03 G01N33/49

    摘要: Known diffusion chambers for measuring the pH value and absorption of aqueous substance samples take measurements with a relatively low resolution, large substance sample quantities, or in a non-simultaneous manner in the same sample. The diffusion chamber (01) according to the invention is equipped with a high-resolution pH optode (07) and a compact spectrophotometer (13), the respective glass fiber optics of which are integrated into the central cylinder (03) of the diffusion chamber (01) by means of special structural measures. In the process, the pH optode (07) is designed in the form of a syringe and is inserted into a sample slide (20), the cannula (11) being inserted into a diagonal groove (32) and guided through a sealing ring (22) on the sample slide (20) in a gas-tight manner. Thus, very small substance samples (05) (

    摘要翻译: 用于测量水溶性物质样品的pH值和吸收率的已知扩散室在相同样品中以相对低分辨率,大物质样品量或以非同时方式进行测量。 根据本发明的扩散室(01)配备有高分辨率pH光电极(07)和紧凑型分光光度计(13),其各自的玻璃纤维光学器件被集成到扩散室的中心圆柱体(03)中 (01)通过特殊的结构措施。 在该过程中,pH光极(07)被设计成注射器的形式并被插入到样品载玻片(20)中,套管(11)被插入对角槽(32)中并被引导通过密封环( 22)以气密方式固定在样品载玻片(20)上。 因此,可以测量非常小的物质样品(05)(<20μl),同时仍然确保测量尖端(28)浸入物质样品(05)的边缘区域(29)中而不中断光束路径(30 )进行吸收测量。 分光光度计的供光和取光光纤被集成到上,下圆柱形光学系统支架(23,25)中,其中样品室(04)通过插入的下光学系统支架(25) 隔圈(27),并确保准直透镜(26)和样品(05)之间的最小距离。 例如,当确定来自非常小的生物体的少量血液的氧结合曲线时,可以使用扩散室(01)。

    HOCHDRUCKFESTE PROBENKAMMER FÜR DIE DURCHLICHT-MIKROSKOPIE UND VERFAHREN ZU DEREN HERSTELLUNG
    5.
    发明公开
    HOCHDRUCKFESTE PROBENKAMMER FÜR DIE DURCHLICHT-MIKROSKOPIE UND VERFAHREN ZU DEREN HERSTELLUNG 有权
    HOCHDRUCKFESTE PROBENKAMMERFÜRDIE DURCHLICHT-MIKROSKOPIE UND VERFAHREN ZU DEREN HERSTELLUNG

    公开(公告)号:EP3234553A1

    公开(公告)日:2017-10-25

    申请号:EP15831042.5

    申请日:2015-12-04

    IPC分类号: G01N21/03 G02B21/34 B01L3/00

    摘要: Known high-pressure-resistant specimen chambers have complex progressive metal seals. Non-high-pressure-resistant specimen chambers have complex clamps for the viewing windows. In the case of the high-pressure-resistant specimen chamber (01) according to the invention, viewing windows (07, 08) having plane-parallel raised areas (23, 25), which protrude into viewing openings (13, 14) of clamping elements (11, 12) in an accurately fitting manner, and high-pressure-resistant seals (09, 10) are provided. Thus, a compact, very well joined high-pressure-resistant specimen chamber results. The elements are composed of a plastomer that can be plastically deformed when high pressure is applied. In the production, flat disks (32, 34) composed of polycarbonate and sealing rings (31, 33) composed of polytetrafluoroethylene are inserted into the specimen chamber (01) and a pressure above the pressure at which plastic deformation starts is applied to the specimen chamber after the final assembly. The flat disks (32, 34) and the sealing rings (31, 33) thereby form the viewing windows (07, 08) and high-pressure-resistant seals (09, 10). The specimen chamber (01) can be used as a parameterizable flow-through chamber for the microscopic in situ observation of living organisms under an overpressure corresponding to the high pressure that is used, said overpressure corresponding to the depth of the sea, e.g., 500 m and more, of the habitat of the organisms.

    摘要翻译: 已知的耐高压样品室具有复杂的渐进式金属密封件。 非耐高压样品室对于观察窗具有复杂的夹具。 在根据本发明的耐高压样品室(01)的情况下,具有平面平行凸起区域(23,25)的观察窗(07,08)突出到观察开口(13,14)中 以精确配合的方式设置夹紧元件(11,12),以及耐高压密封件(09,10)。 因此,结果紧凑,结合良好的耐高压样品室。 这些元素由塑性体组成,在施加高压时可塑性变形。 在制造过程中,将由聚碳酸酯构成的平盘(32,34)和由聚四氟乙烯构成的密封环(31,33)插入到样品室(01)中,并且对样品施加高于开始塑性变形的压力的压力 最后组装后的腔室。 扁平圆盘32,34和密封环31,33由此形成观察窗口07,08和耐高压密封件09,10。 样品室(01)可以用作可参数化的流通室,用于在对应于所使用的高压的超压下对生物进行微观原位观察,所述超压对应于海水的深度,例如500 米和更多,生物的栖息地。