公开(公告)号：EP2451823A2

公开(公告)日：2012-05-16

申请号：EP10797741.5

申请日：2010-07-06

申请人： Alnylam Pharmaceuticals, Inc.

发明人： ROSSOMANDO, Anthony ,  MARAGANORE, JohnM. ,  POLLARD, Stuart ,  KOCISKO, David ,  MANOHARAN, Muthiah ,  HINKLE,Greg ,  BETTENCOURT, Brian ,  HOGAN, Shannon

IPC分类号： C07H21/04 ,  C12N15/11 ,  C12N5/00 ,  C12N5/07

CPC分类号： C07K16/00 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/3515

摘要： The invention provides compositions and methods for producing a biological product from a host cell. In various embodiments, the biological product is a polypeptide, a metabolite, a nutraceutical, a chemical intermediate, a biofuel, a food additive, or an antibiotic. In one aspect, the invention provides for a method for producing a biological product from a host cell. The method generally comprises contacting the cell with a RNA effector molecule, a portion of which is complementary to a target gene, maintaining the cell in a large-scale bioreactor for a time sufficient to modulate expression of the target gene, wherein the modulation enhances production of the biological product from the cell, and isolating the biological product from the cell.