公开(公告)号：EP1434999A2

公开(公告)日：2004-07-07

申请号：EP02739342.0

申请日：2002-05-20

申请人： Amersham Biosciences AB

发明人： BHIKHABHAI, Ramagauri,c/o Amersham Biosciences AB ,  LIMINGA, Maria,c/o Amersham Biosciences AB ,  MALOISEL, Jean-Luc ,  PALMGREN, Ronnie ,  KEOUGH, Thomas W.,The Procter and Gamble Comp. ,  YOUNGQUIST, Robert S.,The Procter and Gamble Comp ,  VAUGHN, Harold L.,c/o The Procter and Gamble Comp ,  YELM, Ken E.,c/o The Procter and Gamble Company

IPC分类号： G01N33/58

CPC分类号： G01N33/6848 ,  G01N33/58 ,  G01N33/6824 ,  G01N33/6851

摘要： The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.