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公开(公告)号：EP1664759A1
公开(公告)日：2006-06-07
申请号：EP04775378.5
申请日：2004-09-06
CPC分类号： B01J20/3278 , B01D15/32 , B01D15/362 , B01D15/38 , B01D15/424 , B01J20/286 , B01J20/288 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/328
摘要： The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.
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2.发明公开
公开(公告)号：EP1599265A1
公开(公告)日：2005-11-30
申请号：EP04717913.0
申请日：2004-03-05
发明人： JOHANSSON, Bo-Lennart,Amersham Biosciences Ab , MALOISEL, Jean-Luc,Amersham Biosciences Ab , THEVENIN, Nicolas,Amersham Biosciences Ab
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
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公开(公告)号：EP1558927A1
公开(公告)日：2005-08-03
申请号：EP03791513.9
申请日：2003-08-18
发明人： Berg, Hans , Busson, Philippe , Carlsson, Mats
CPC分类号： B01D15/363 , B01D15/1807 , B01J20/285 , B01J20/286 , B01J20/3285 , B01J20/3293 , B01J39/26 , B01J41/20
摘要： The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washing said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic binding groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.
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4.发明公开Method and device for producing droplets from a coherent layer of even thickness of liquid or melt on a rotating disk 有权用于从液体相干或产生微滴的方法和装置熔融均匀厚度的层在一个旋转盘
公开(公告)号：EP1502654A3
公开(公告)日：2005-04-27
申请号：EP04025910.3
申请日：2000-11-29
IPC分类号： B05B3/10
CPC分类号： B01J2/02 , B05B3/1007
摘要： Method for continuously producing droplets by producing a coherent layer of a liquid/melt of even thickness by means of centrifugal action along the entire periphery of a rotating disk (13, 113), liquid/melt being supplied to the disk around its entire axis of rotation from means (12, 112) and leaving the disk as individual droplets which are then collected. The means (12, 112) distributing the liquid/melt onto the disk (13, 113) has according to the invention essentially the same speed and direction of rotation as the disk, and the liquid/melt undergoes under the influence of centrifugal action circumferentially a spreading and change in velocity across the disk (13, 113) in contact with the same.
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公开(公告)号：EP1518114A1
公开(公告)日：2005-03-30
申请号：EP03733764.9
申请日：2003-06-18
CPC分类号： B01J20/286 , B01D15/327 , B01D15/361 , B01D15/3804 , B01J20/26 , B01J20/265 , B01J20/285 , B01J20/3272 , B01J20/3274 , B01J39/26 , B01J41/20 , C08B37/00 , C08G81/00 , C08J7/12
摘要： The present invention is a surface-modified base matrix comprised of a porous polymeric base matrix onto which branched hydrophilic polyhydroxy-functional polymers have been covalently attached, wherein the polyhydroxy-functional polymers are hyperbranched polymers presenting a degree of branching (DB) of at least about 0.2 and each polymer is tethered to the base matrix at two or more points. The present matrix can for example be a cross-linked carbohydrate material, such as agarose, and the hyperbranched hydrophilic polymer can e.g. be a copolymer of epichiorohydrin and a sugar. The invention also relates to a method of surface-modification of a porous base matrix by activating functional hydroxy groups thereon and contacting the activated matrix with a hydrophilic hyperbranched hydroxy-functional polymer.
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公开(公告)号：EP1504031A1
公开(公告)日：2005-02-09
申请号：EP03725953.8
申请日：2003-05-09
发明人： BELEW, MakonnenAmersham Biosciences AB , LI, Mei, YanNorth China Pharmaceutical Group Corp , ZHANG, WeiNorth China Pharmaceutical Group Corp.
IPC分类号： C07K14/765 , C07K1/16
CPC分类号： C07K14/765
摘要： The present invention is a method of purifying recombinant human serum albumin (rHSA) from a solution, which method comprises to subject a cell culture supernatant (CCS) comprising rHSA cation exchange on a bimodal high salt tolerant matrix; hydrophobic interaction chromatography (HIC); and anion exchange. The bimodal high salt tolerant cation exchange matrix used enables per-forming a purification of a cell culture supernatant directly, in the sense that no further dilution thereof is necessary. Due to its high binding capacity, said bimodal cation exchange matrix also allows use of a smaller amount of matrix as compared to a corresponding conventional cation exchanger matrix. Accordingly, the present invention allows substantial savings as regards volumes and consequently operation costs.
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公开(公告)号：EP1200186B1
公开(公告)日：2004-10-20
申请号：EP00927160.2
申请日：2000-05-08
发明人： BERG, Hans , CARLSSON, Mats
CPC分类号： B01J20/286 , B01J20/26 , B01J20/28004 , B01J20/28014 , B01J20/28016 , B01J20/28023 , B01J20/28026 , B01J20/28042 , B01J20/28045 , B01J20/28085 , B01J20/28092 , B01J20/28095 , B01J20/28097 , B01J20/285 , B01J2220/52
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公开(公告)号：EP1455920A1
公开(公告)日：2004-09-15
申请号：EP02793661.6
申请日：2002-12-17
CPC分类号： B01J20/262 , B01D15/368 , B01D15/3847 , B01J20/265 , B01J20/285 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3253 , B01J20/3255 , C07K1/16
摘要： The present invention relates to a method of separating a compound from a liquid, which method comprises providing a separation matrix comprising at least one uncharged ligand; providing a liquid wherein the compound to be separated is present in a positively charged state; contacting said matrix with said liquid to adsorb the compound; and removing the liquid. The uncharged ligands possess a quadrupole or dipole moment, allowing for a cation-p interaction between the compound and the ligand. The present invention also encompasses the use of a separation matrix, which comprises an uncharged group that possesses a quadrupole or dipole moment, in said method.
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公开(公告)号：EP1434999A2
公开(公告)日：2004-07-07
申请号：EP02739342.0
申请日：2002-05-20
发明人： BHIKHABHAI, Ramagauri,c/o Amersham Biosciences AB , LIMINGA, Maria,c/o Amersham Biosciences AB , MALOISEL, Jean-Luc , PALMGREN, Ronnie , KEOUGH, Thomas W.,The Procter and Gamble Comp. , YOUNGQUIST, Robert S.,The Procter and Gamble Comp , VAUGHN, Harold L.,c/o The Procter and Gamble Comp , YELM, Ken E.,c/o The Procter and Gamble Company
IPC分类号： G01N33/58
CPC分类号： G01N33/6848 , G01N33/58 , G01N33/6824 , G01N33/6851
摘要： The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.
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