公开(公告)号：EP1599265A1

公开(公告)日：2005-11-30

申请号：EP04717913.0

申请日：2004-03-05

申请人： Amersham Biosciences AB

发明人： JOHANSSON, Bo-Lennart,Amersham Biosciences Ab ,  MALOISEL, Jean-Luc,Amersham Biosciences Ab ,  THEVENIN, Nicolas,Amersham Biosciences Ab

IPC分类号： B01D15/08 ,  B01J20/22 ,  B01J20/30

CPC分类号： B01J20/3285 ,  B01D15/325 ,  B01D15/327 ,  B01J20/286

摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.