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    Assembly for thermal ycler for PCR
    3.
    发明公开
    Assembly for thermal ycler for PCR 失效
    BaugruppefürThermozykliergerätfürPCT

    公开(公告)号：EP2186568A1

    公开(公告)日：2010-05-19

    申请号：EP09016131.6

    申请日：1998-03-30

    申请人： Applera Corporation

    发明人： Atwood, John G. ,  Fawcett, Adrian ,  Ferrara, Keith S ,  Hetherington, Paul M. ,  Noreiks, Richard W. ,  Olsen, Douglas E. ,  Widomski, John R. ,  Wittmer, Charles M.

    IPC分类号： B01L7/00

    CPC分类号： B01L7/52 ,  B01L3/50853 ,  B01L2300/045 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01R27/205 ,  G05D23/1919 ,  H01L35/30

    摘要： An assembly is provided comprising a sample block configured to receive a plurality of sample vials, a plurality of Peltier thermal electric devices, a heat sink, and a memory device that stores data related to the assembly. Moreover, a system for performing polymerase chain reaction in a plurality of liquid reaction mixtures is provided, comprising a plurality of vials for containing a plurality of liquid reaction mixtures, the plurality of vials having an upper portion and a lower portion, such an assembly, the assembly being configured to cycle the plurality of vials through a series of temperature excursions, a cover configured to apply a seating force directly to the plurality of vials and to apply heat to the upper portion of the plurality of vials, and a computing apparatus configured to control the temperature excursions of the assembly and the heating of the cover. Moreover, a polymerase chain reaction device is provided, comprising such an assembly, wherein the assembly is configured to be removed from the polymerase chain reaction device. Finally, a method is provided of calibrating an assembly for cycling samples through a series of temperature excursions comprising a sample block for receiving vials, a plurality of Peltier thermal electric devices, a heat sink, and a memory device capable of storing data related to the assembly, the method comprising: applying power to the plurality of Peltier thermal electric devices, causing the assembly to cycle through a desired series of temperature excursions; measuring the actual temperature excursions; comparing the actual temperature excursions with the desired temperature excursions; adjusting the power applied to the plurality of Peltier thermal electric devices so that the actual temperature excursions match the desired temperature excursions; and recording data in the memory device for further utilization in obtaining said desired series of temperature excursions.

    摘要翻译： 提供了一种组件，其包括被配置为接收多个样品瓶的样品块，多个珀耳帖热电装置，散热器和存储与组件相关的数据的存储装置。 此外，提供了一种用于在多个液体反应混合物中进行聚合酶链反应的系统，包括用于容纳多个液体反应混合物的多个小瓶，所述多个小瓶具有上部和下部， 所述组件被配置为使所述多个小瓶循环通过一系列温度偏移，盖子被配置成直接施加到所述多个小瓶中并将热量施加到所述多个小瓶的上部;以及计算设备 以控制组件的温度偏移和盖的加热。 此外，提供了聚合酶链式反应装置，其包括这样的组件，其中组件被配置为从聚合酶链式反应装置中移除。 最后，提供了一种校准用于循环样品通过一系列温度偏移的组件的方法，所述温度偏移包括用于接收小瓶的样品块，多个珀耳帖热电装置，散热器和能够存储与 该方法包括：向所述多个珀尔帖热电装置施加电力，使所述组件循环通过期望的一系列温度偏移; 测量实际温度偏差; 将实际温度偏移与期望的温度偏差进行比较; 调整施加到多个珀尔帖热电装置的功率，使得实际温度偏移与期望的温度偏移匹配; 以及将数据记录在所述存储器装置中，以进一步利用以获得所述期望的一系列温度偏移。