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    Buffers for electrophoresis and use thereof
    2.
    发明公开
    Buffers for electrophoresis and use thereof 审中-公开
    河豚z。。。。。

    公开(公告)号：EP2196799A2

    公开(公告)日：2010-06-16

    申请号：EP10150349.8

    申请日：2002-07-19

    申请人： Applera Corporation

    发明人： Hacker, Kevin ,  Voss, Karl

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44747

    摘要： Buffer compositions with or without sieving formulations are useful when used in connection with a capillary electrophoresis system as shown in Figure 1 . The buffer composition can include Bis-Tris, TAPS and/or TAPSO and optionally a chelating agent such as EDTA. Methods of separating samples containing DNA or RNA with the capillary electrophoresis system are also described.

    摘要翻译： 当与如图1所示的毛细管电泳系统结合使用时，具有或不具有筛分制剂的缓冲液组合物是有用的。缓冲液组合物可包括Bis-Tris，TAPS和/或TAPSO以及任选的螯合剂如EDTA。 还描述了用毛细管电泳系统分离含有DNA或RNA的样品的方法。

    MALDI PLATE WITH REMOVABLE INSERT
    5.
    发明授权
    MALDI PLATE WITH REMOVABLE INSERT 有权
    具有可拆卸应用MALDI-PLATE

    公开(公告)号：EP1673169B1

    公开(公告)日：2009-12-30

    申请号：EP04794366.7

    申请日：2004-10-07

    申请人： Applera Corporation

    发明人： HUTCHINS, Timothy, E. ,  TOMLINSON, Andrew, J. ,  SAVICKAS, Philip, J.

    IPC分类号： B01L3/00

    CPC分类号： H01J49/0418 ,  B01L9/52 ,  B01L2200/025 ,  B01L2300/0819 ,  B01L2300/0822

    摘要： A sample plate structure is provided including a retainer plate having a central opening, a sample insert plate which fits into the opening, an optional pressure plate and a handle. The handle applies pressure to the sample insert plate to retain it within the retainer plate. The sample insert plate is provided with a peripheral configuration, which assures that the sample insert is properly oriented within the sample plate support structure and is held flat. While the sample insert plate can be a consumable, the remaining portion of the apparatus can be reused.

    Improvements in thermal cycler for PCR
    6.
    发明公开
    Improvements in thermal cycler for PCR 失效
    在用于PCR的热电路的改进

    公开(公告)号：EP2090366A3

    公开(公告)日：2009-11-18

    申请号：EP09006229.0

    申请日：1998-03-30

    申请人： Applera Corporation

    发明人： Atwood, John, G. ,  Fawcett, Adrain ,  Ferrara, Keith, S. ,  Hetherington, Paul, M. ,  Noreiks, Richard W. ,  Olsen, Douglas, E. ,  Widomski, John, R. ,  Wittmer, Charles, M.

    IPC分类号： B01L7/00

    CPC分类号： B01L7/52 ,  B01L3/50853 ,  B01L2300/045 ,  B01L2300/0829 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01R27/205 ,  G05D23/1919 ,  H01L35/30

    摘要： A method for calibrating a removable assembly for cycling samples through a series of temperature excursions is disclosed. The removable assembly is attached to an instrument and comprises a sample block for receiving vials, a plurality of thermoelectric devices, a heat sink, a clamping mechanism positioned so as to clamp the thermoelectric devices between the sample block and the heatsink, and a memory device capable of storing data related to the removable assembly. The method comprises the steps of: applying power to the thermoelectric devices, causing the removable assembly to cycle through a desired series of temperature excursions, measuring the actual temperature excursions, comparing the actual temperature excursions with the desired temperature excursions, calibrating the removable assembly by adjusting the power applied to the thermoelectric devices so that the actual temperature excursions match the desired temperature excursions and recording the adjusted power in the memory device for further utilization in obtaining the desired series of temperature excursions, to form a calibrated removable assembly, removing the calibrated removable assembly from the instrument, and attaching the removable assembly to a different instrument.

    MULTIPLE ENZYME ASSAYS
    7.
    发明授权
    MULTIPLE ENZYME ASSAYS 有权
    多酶检测方法

    公开(公告)号：EP1151090B1

    公开(公告)日：2009-09-02

    申请号：EP99965243.1

    申请日：1999-12-14

    申请人： Applera Corporation

    发明人： BRONSTEIN, Irena ,  MARTIN, Christopher ,  OLESEN, Corinne ,  VOYTA, John ,  YAN, Yu-Xin

    IPC分类号： C12Q1/68 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/66 ,  G01N33/50

    CPC分类号： C12Q1/6897 ,  C12Q1/00 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/66 ,  G01N2333/916 ,  G01N2333/924 ,  G01N2333/95

    摘要： The present invention discloses multiple enzyme assays which measure the activity of at least one endogenous enzyme in a single aliquot and a method of measuring the activity of multiple enzymes in an aliquot of a sample extract, wherein at least one of the enzymes is an endogenous enzyme. In one embodiment of the invention the activity of a first enzyme is quantified by measuring the light signal produced by degradation of a first enzyme substrate by the first enzyme and the activity of the second enzyme is quantified by measuring the light signal produced by the degradation of a second substrate. In the method of the present invention, both quantifications are performed on the same aliquot of sample extract. Different embodiments of the present invention provide for the detection of more than one endogenous enzyme and for the detection of at least one reporter enzyme and at least one endogenous enzyme. The present invention also discloses kits for detecting the activity of multiple enzymes.

    DINUCLEOTIDE MRNA CAP ANALOGS
    9.
    发明公开
    DINUCLEOTIDE MRNA CAP ANALOGS 审中-公开
    DINUKLEOTID体mRNA-CAP-ANALOGA

    公开(公告)号：EP2049665A2

    公开(公告)日：2009-04-22

    申请号：EP07810384.3

    申请日：2007-07-10

    申请人： Applera Corporation

    发明人： KORE, Anilkumar, R. ,  SHANMUGASUNDARAM, Muthian

    IPC分类号： C12N15/11 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68

    CPC分类号： C07H21/02 ,  C07H21/04 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/317 ,  C12N2320/51

    摘要： Novel cap analogs which are easily synthesized, resulting in high levels of capping efficiency and transcription and improved translation efficiencies are provided. Such caps are methylated at the N7 position of one or both guanosines of the dinucleotide cap as well as at the 3' position on the ribose ring. Substituent groups on the ribose ring also result in the cap being incorporated in the forward orientation. Also provided are methods useful for preparing capped analogs and using mRNA species containing such analogs are also contemplated herein, as well as kits containing the novel cap analogs.