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1.发明公开5-BROMO-2'-DEOXY-URIDINE LABELED NUCLEOTIDE TRIPHOSPHATES AND NUCLEIC ACID PROBES AND METHODS OF MAKING AND USING THE SAME 有权5-溴-2'-脱氧尿苷标记的核苷三磷酸和核酸探针及其制备和使用方法
公开(公告)号：EP2170924A2
公开(公告)日：2010-04-07
申请号：EP08770962.2
申请日：2008-06-13
申请人： Applera Corporation
发明人： KORE, Anilkumar, R. , HU, Zhongting
CPC分类号： C07H21/00
摘要： 5-bromo-2'-deoxy-uridine (BrdU) labeled nucleotide triphosphates and nucleic acid probes are described herein. The BrdU labeled nucleotide triphosphates include a linker between the nucleotide triphosphate and the BrdU moiety. The linker can be cleavable or non-cleavable. The nucleotide triphosphates can be a ribonucleotide triphosphates, 2'-deoxyribonucleotide triphosphates or 2',3 '-dideoxyribonucleotide triphosphates. The nucleic acid probes can be used for in situ hybridization.
摘要翻译： 本文描述了5-溴-2'-脱氧尿苷（BrdU）标记的三磷酸核苷酸和核酸探针。 BrdU标记的核苷酸三磷酸酯包括三磷酸核苷酸和BrdU部分之间的接头。 接头可以是可切割的或不可切割的。 三磷酸核苷酸可以是三磷酸核糖核苷酸，2'-脱氧核糖核苷酸三磷酸或2'，3'-二脱氧核糖核苷酸三磷酸。 核酸探针可用于原位杂交。
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公开(公告)号：EP2049665A2
公开(公告)日：2009-04-22
申请号：EP07810384.3
申请日：2007-07-10
申请人： Applera Corporation
CPC分类号： C07H21/02 , C07H21/04 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51
摘要： Novel cap analogs which are easily synthesized, resulting in high levels of capping efficiency and transcription and improved translation efficiencies are provided. Such caps are methylated at the N7 position of one or both guanosines of the dinucleotide cap as well as at the 3' position on the ribose ring. Substituent groups on the ribose ring also result in the cap being incorporated in the forward orientation. Also provided are methods useful for preparing capped analogs and using mRNA species containing such analogs are also contemplated herein, as well as kits containing the novel cap analogs.
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