公开(公告)号：EP1354062A2

公开(公告)日：2003-10-22

申请号：EP01971029.2

申请日：2001-09-13

申请人： Archemix Corporation

发明人： STANTON, Marty ,  EPSTEIN, David ,  HAMAGUCHI, Nobuko

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  B01J2219/00385 ,  B01J2219/00479 ,  B01J2219/00608 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00662 ,  B01J2219/00691 ,  B01J2219/00722 ,  B82Y30/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/485 ,  C12Q1/6825 ,  C40B40/06 ,  C40B60/14 ,  C12Q2565/101 ,  C12Q2525/179 ,  C12Q2521/337 ,  C12Q2521/501

摘要： Methods for engineering a target activated biosensor are provided. Biosensors comprise a plurality of nucleic acid sensor molecules labeled with a first signaling moiety and a second signaling moiety. The nucleic acid sensor molecules recognizes target molecules which do not naturally bind to DNA. Binding of a target molecule to the sensor molecules triggers a change in the proximity of the signaling moieties which leads to a change in the optical properties of the nucleic acid sensor molecules on the biosensor. Reagents and systems for performing the method are also provided. The method is useful in diagnostic applications and drug optimization.