公开(公告)号：EP3259354A1

公开(公告)日：2017-12-27

申请号：EP16753010.4

申请日：2016-02-17

申请人： California Institute of Technology

发明人： SUN, Zachary Z. ,  MURRAY, Richard M. ,  SINGHAL, Vipul

IPC分类号： C12N15/09 ,  C12N15/113 ,  C12P1/00

CPC分类号： G06F19/12 ,  C12N15/1075 ,  C12N15/1086 ,  C12N15/1089 ,  C12N15/1093 ,  C12N15/52 ,  C12Q1/6897 ,  C40B30/02 ,  C12Q2521/119 ,  C12Q2521/337 ,  C12Q2537/165

摘要： Provided herein are methods and arrangements and related cell-free biomolecular breadboards configured to design, build, implement, debug, and/or test a genetic circuit to be operated in a target environment, by testing in a cell-free system under conditions of the target environment, molecular components of the genetic circuit and/or combinations thereof to select the molecular components and/or combinations thereof of a genetic circuit operative in the target environment.

公开(公告)号：EP2861770A1

公开(公告)日：2015-04-22

申请号：EP13807047.9

申请日：2013-06-18

申请人： SpeeDx Pty Ltd

发明人： BONE, Simon Mark ,  TODD, Alison Velyian ,  MEEHAN, Timothy Daniel

IPC分类号： C12Q1/68 ,  C12N9/22 ,  C12Q1/44

CPC分类号： C12Q1/6823 ,  C12N9/22 ,  C12Q1/682 ,  G01N2333/9005 ,  C12Q2521/337 ,  C12Q2521/345 ,  C12Q2525/205 ,  C12Q2537/163 ,  C12Q2565/1025

摘要： The present invention relates to compositions and methods for the detection of target molecules, and the amplification of detectable signals generated by detection assays. More specifically, the present invention relates to methods utilizing catalytic nucleic acid enzymes to generate and/or amplify a signal indicative of the presence of target molecules (e.g. nucleic acids and proteins), and compositions for use in the methods.

摘要翻译： 本发明涉及用于检测靶分子的组合物和方法，以及通过检测分析产生的可检测信号的扩增。 更具体地，本发明涉及利用催化性核酸酶产生和/或扩增指示靶分子（例如核酸和蛋白质）存在的信号的方法，以及用于该方法的组合物。

公开(公告)号：EP2142673B1

公开(公告)日：2013-05-22

申请号：EP08733324.1

申请日：2008-04-04

申请人： Johnson & Johnson Research Pty Limited

发明人： TODD, Alison, Velyian ,  MOKANY, Elisa ,  DOAN, Tram, Bich ,  YOUNG, Paul, Ean ,  SWAIN, Helen, Anne

IPC分类号： C12Q1/68 ,  C12N9/00 ,  C12N15/09

CPC分类号： C12Q1/6811 ,  C12N15/111 ,  C12N2310/127 ,  C12N2320/11 ,  C12Q1/6816 ,  G01N2333/9005 ,  C12Q2565/1015 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2521/501 ,  C12Q2521/337 ,  C12Q2521/301 ,  C12Q2537/163 ,  C12Q2537/162

公开(公告)号：EP2142673A4

公开(公告)日：2010-10-27

申请号：EP08733324

申请日：2008-04-04

申请人： JOHNSON & JOHNSON RES PTY LTD

发明人： TODD ALISON VELYIAN ,  MOKANY ELISA ,  DOAN TRAM BICH ,  YOUNG PAUL EAN ,  SWAIN HELEN ANNE

IPC分类号： C12Q1/68 ,  C12N9/00 ,  C12N15/09

CPC分类号： C12Q1/6811 ,  C12N15/111 ,  C12N2310/127 ,  C12N2320/11 ,  C12Q1/6816 ,  G01N2333/9005 ,  C12Q2565/1015 ,  C12Q2537/149 ,  C12Q2537/143 ,  C12Q2521/501 ,  C12Q2521/337 ,  C12Q2521/301 ,  C12Q2537/163 ,  C12Q2537/162

公开(公告)号：EP1025266B1

公开(公告)日：2009-05-20

申请号：EP99917023.6

申请日：1999-03-16

申请人： JOHNSON & JOHNSON RESEARCH PTY LIMITED

发明人： TODD, Alison, V. ,  FUERY, Caroline, J. ,  CAIRNS, Murray, J.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q1/683 ,  C12Q1/6853 ,  C12Q1/6858 ,  C12Q2521/337

摘要： This invention provides methods and kits useful for determining whether a subject is afflicted with a disorder characterized by the presence of one or more known nucleic acid mutations. The instant methods comprise steps of nucleic acid molecule isolation, amplification, contact with one or more catalytic nucleic acid molecules specifically cleaving a target sequence present either in the case of disorder or wild-type, but not both, and determining cleavage of the amplified segment(s).

公开(公告)号：EP1987166A2

公开(公告)日：2008-11-05

申请号：EP07868171.5

申请日：2007-02-04

申请人： Columbia University

发明人： KOLPASHCIKOV, Dmitry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q2525/161 ,  C12Q2521/337

摘要： New binary deoxyribozyme or ribozyme probes and methods are described for nucleic acid analysis, which allows the detection of nucleic acids under mild physiologic conditions with extraordinary specificity and high sensitivity to single nucleotide mismatches without PCR amplification.

公开(公告)号：EP1871896A2

公开(公告)日：2008-01-02

申请号：EP06706144.0

申请日：2006-04-10

申请人： In Situ RCP A/S Under Founding

发明人： KOCH, Jørn ,  STOUGAARD, Magnus ,  LOHMANN, Jakob Schwalbe

IPC分类号： C12Q1/68

CPC分类号： C12Q1/682 ,  C12Q1/6816 ,  C12Q2531/125 ,  C12Q2521/101 ,  C12Q2521/325 ,  C12Q2525/307 ,  C12Q2525/301 ,  C12Q2521/337 ,  C12Q2525/155 ,  C12Q2521/501

摘要： The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3'end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.

公开(公告)号：EP1844160A2

公开(公告)日：2007-10-17

申请号：EP06709601.6

申请日：2006-02-01

申请人： Enigma Diagnostics Ltd.

发明人： SQUIRRELL, David, James ,  LEE, Martin, Alan

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848 ,  C12Q2525/301 ,  C12Q2521/337

摘要： A method for adding a first and a second functional nucleic acid sequence to a reaction mixture, in particular an amplification reaction mixture in a predetermined stoichiometry and/or at a predetermined point in time, said method comprising adding to the reaction mixture an oligonucleotide comprising a first and a second functional nucleic acid sequence separated by a spacer sequence, said spacer sequence comprising a region which, when double stranded, comprises a cleavable region, forming a cleavable double stranded region within the spacer region of said oligonucleotide, and cleaving the double stranded region within said oligonucleotide. Oligonucleotides for use in the method, and comprising a first and a second functional nucleic acid sequence, such as primers or probes used in an amplification reaction, separated by a spacer sequence, is also provided.

公开(公告)号：EP1504100A1

公开(公告)日：2005-02-09

申请号：EP03714277.5

申请日：2003-03-18

申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS

发明人： LU, Yi ,  LIU, Juewen

IPC分类号： C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q1/6825 ,  Y10S436/80 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/501 ,  C12Q2563/137 ,  C12Q2521/337 ,  C12Q2541/101

摘要： A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions.

公开(公告)号：EP1327683A3

公开(公告)日：2004-01-07

申请号：EP03075150.7

申请日：1995-05-15

申请人： The Government of the United States of America, as represented by the Secretary Department of Health and Human Services ,  Northern Illinois University

发明人： Hampel, Arnold ,  Dipaolo, Joseph ,  Siwkowski, Andrew

IPC分类号： C12N15/52 ,  C12N9/00 ,  A61K31/70 ,  C12Q1/68 ,  C12N15/11 ,  C12N5/10 ,  A61K31/713 ,  A61P35/00

CPC分类号： C12N15/1131 ,  A61K38/00 ,  A61K48/00 ,  C12N2310/111 ,  C12N2310/122 ,  C12N2799/027 ,  C12Q1/683 ,  C12Q1/708 ,  C12Q2521/337

摘要： The present invention is related to synthetic ribozymes, i.e. ribozymes including a hairpin portion, binding sites for binding to a human papilloma virus (HPV) after viral base 419 or 434, respectively, and cleavage sites for cleaving HPV virus are disclosed. The invention also includes a diagnostic assay for determining the presence of HPV-16 in tissues based on PCR amplification.