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公开(公告)号：EP3372677B1
公开(公告)日：2019-08-21
申请号：EP16861871.8
申请日：2016-10-05
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公开(公告)号：EP2924114B1
公开(公告)日：2016-12-07
申请号：EP13856565.0
申请日：2013-09-05
发明人： YANAGIDA, Koichiro
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2750/14351
摘要： A method for stably and easily producing a parvovirus having a high infectivity titer is provided. The problem is solved by a method for producing a parvovirus having an infectivity titer as high as 10 8 TCID 50 /mL or more in a culture supernatant, comprising the steps of inoculating a seed virus of the parvovirus into a culture substrate comprising host cells having a cell density of 1/500 to 1/20 of the cell density of the host cells confluently grown and a medium at a multiplicity of infection of 0.0001 to 0.1, culturing for a period of 5 to 11 times the doubling time of the host cells, and recovering a culture supernatant.
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公开(公告)号：EP4112735A1
公开(公告)日：2023-01-04
申请号：EP21761452.8
申请日：2021-02-26
IPC分类号： C12Q1/34 , C12Q1/6851 , C12Q1/686 , C12Q1/70 , G01N33/569
摘要： Provided is a method for evaluating the viral clearance capability of a virus removal medium, comprising the steps of: (a) adding a viral capsid-containing liquid to a solution to be purified; (b) contacting the virus removal medium with the solution to be purified that has been supplemented with the viral capsid-containing liquid to harvest a purified solution; and (c) quantifying a total viral capsid in the solution to be purified before the purification and a total viral capsid in the purified solution.
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公开(公告)号：EP3372677A1
公开(公告)日：2018-09-12
申请号：EP16861871.8
申请日：2016-10-05
CPC分类号： C12N7/00 , C12N7/02 , C12N2750/14351
摘要： Provided are a parvovirus derived from an unconcentrated cell culture supernatant, having a infectivity titer of 10 9 TCID 50 /mL or more and an {infectivity titer (TCID 50 /mL)}:{impurity protein concentration (ng/mL)} ratio more than 5000:1; and a method of producing such a high-infectivity titer and high-purity parvovirus.
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5.发明公开METHOD FOR PRODUCING PARVOVIRUS HAVING HIGH INFECTIVITY TITER 有权VERFAHREN ZUR HERSTELLUNG EINES PARVOVIRUS MIT EINEMHOCHINFEKTIÖSEMTITER
公开(公告)号：EP2924114A1
公开(公告)日：2015-09-30
申请号：EP13856565.0
申请日：2013-09-05
发明人： YANAGIDA, Koichiro
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2750/14351
摘要： A method for stably and easily producing a parvovirus having a high infectivity titer is provided. The problem is solved by a method for producing a parvovirus having an infectivity titer as high as 10 8 TCID 50 /mL or more in a culture supernatant, comprising the steps of inoculating a seed virus of the parvovirus into a culture substrate comprising host cells having a cell density of 1/500 to 1/20 of the cell density of the host cells confluently grown and a medium at a multiplicity of infection of 0.0001 to 0.1, culturing for a period of 5 to 11 times the doubling time of the host cells, and recovering a culture supernatant.
摘要翻译： 提供了一种稳定且容易地产生感染性高滴度的细小病毒的方法。 该问题通过在培养上清液中产生感染效价高达10 8 TCID 50 / mL以上的细小病毒的方法来解决，其包括以下步骤：将细小病毒的种子病毒接种到包含具有 细胞密度为融合生长的宿主细胞的细胞密度的1/500至1/20，感染复数为0.0001至0.1的培养基，培养宿主细胞的倍增时间的5至11倍 ，并回收培养上清液。
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公开(公告)号：EP2281624A1
公开(公告)日：2011-02-09
申请号：EP09750327.0
申请日：2009-04-30
发明人： YANAGIDA, Koichiro
CPC分类号： B01D61/145 , A61L2/0017 , B01D61/22 , B01D67/0093 , B01D69/02 , B01D69/08 , B01D69/12 , B01D71/34 , B01D71/68 , B01D2323/02 , B01D2323/38 , B01D2325/04 , B01D2325/20 , B01D2325/36 , C07K1/34
摘要： Provided is a filtration method for the step of removing viruses in a process of producing a protein preparation during which an intermediate protein preparation with a high protein concentration flows at high pressure to a virus removal filter. The filtration method employs a virus removal filter which has a close fitting nozzle of which the inlet at least has a pressure resistance of 600 kPa or more, and an effective area of the virus removal membrane of at least 0.0001 m 2 and no more than 0.03 m 2 . An intermediate protein preparation with the protein concentration raised to at least 20 mg/ml and no more than 100 mg/ml by means of a purification process flows down to said virus removal filter under conditions in which the inlet pressure of the intermediate protein preparation is at least 150kPa and no more than 600kPa, the average protein filtration speed is at least 1.0 kg/m 2 /hr, and the filtration time is at least 1 hour and no more than 5 hours, to give an intermediate protein preparation filtrate with a viral removal rate (LRV) of ≥3.
摘要翻译： 本发明提供了在蛋白质制备过程中除去病毒的步骤的过滤方法，其中蛋白质浓度高的中间蛋白质制剂在高压下流向病毒去除过滤器。 过滤方法采用病毒去除过滤器，其具有紧密喷嘴，入口至少具有600kPa以上的耐压性，病毒去除膜的有效面积为至少0.0001m 2且不大于0.03 m 2。 通过纯化过程将蛋白质浓度升高至至少20mg / ml且不超过100mg / ml的中间体蛋白质制剂在中间体蛋白质制剂的入口压力为 至少150kPa且不超过600kPa，平均蛋白质过滤速度至少为1.0kg / m 2 /小时，过滤时间为至少1小时且不超过5小时，得到具有 病毒清除率（LRV）‰¥3。
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