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    METHOD OF CLEAVING POLYPEPTIDE BY USING OmpT PROTEASE MUTANT
    1.
    发明公开
    METHOD OF CLEAVING POLYPEPTIDE BY USING OmpT PROTEASE MUTANT 有权
    VERFAHREN ZUR SPALTUNG VON POLYPEPTID UNTER VERWENDUNG EINER OMPT-PROTEASEMUTANTE

    公开(公告)号：EP1674567A1

    公开(公告)日：2006-06-28

    申请号：EP04773628.5

    申请日：2004-09-29

    申请人： Daiichi Asubio Pharma Co., Ltd.

    发明人： OKUNO, Kazuaki ,  YABUTA, Masayuki

    IPC分类号： C12N15/09 ,  C12P21/02

    CPC分类号： C12P21/06 ,  C07K14/585 ,  C07K14/63 ,  C07K14/695 ,  C12N9/52

    摘要： A polypeptide cleavage method characterized in that arginine or lysine is at the P1 position of a desired cleavage site in a polypeptide, an amino acid other than aspartic acid, glutamic acid or proline is at the P1' position, a single basic amino acid or two or three consecutive basic amino acids are situated at any site in the amino acid sequence from the P10 position to the P3 position or from the P3' position to the P5' position (with the proviso that a single basic amino acid is not situated at the P6 or P4 position), and OmpT protease or its variant enzyme having a substitution at the 97th amino acid from the N-terminus is used to cleave the desired cleavage site in the polypeptide.

    摘要翻译： 一种多肽切割方法，其特征在于精氨酸或赖氨酸位于多肽中所需切割位点的P1位置，除天冬氨酸，谷氨酸或脯氨酸以外的氨基酸位于P1'位置，单碱性氨基酸或二 或三个连续的碱性氨基酸位于从P10位置到P3位置或从P3'位置到P5'位置的氨基酸序列中的任何位置（条件是单个碱性氨基酸不位于 P6或P4位置），并且使用在N末端具有第97位氨基酸取代的OmpT蛋白酶或其变体酶来切割多肽中所需的切割位点。