公开(公告)号：EP4426717A1

公开(公告)日：2024-09-11

申请号：EP22818974.2

申请日：2022-11-04

申请人： The Broad Institute, Inc. ,  President And Fellows Of Harvard College

发明人： LIU, David, R. ,  BLUM, Travis, R. ,  MCCREARY, Julia

IPC分类号： C07K14/33 ,  C12N15/00

CPC分类号： C07K14/33 ,  C12N15/00 ,  C12N9/52 ,  C12Y304/24069 ,  C12Y304/22036 ,  C12R2001/14520210501 ,  C12P21/06 ,  C12N9/6472 ,  A61K38/00

公开(公告)号：EP4373288A1

公开(公告)日：2024-05-29

申请号：EP22755107.4

申请日：2022-07-21

申请人： NAWTER TECH, S.L.U.

发明人： JUAREZ MOLINA, Jesús ,  FERNANDEZ MARTIN, Félix ,  PARRADO RUBIO, Juan ,  ORTS GOMEZ, Ángel ,  MARTINEZ RUIZ, Jesús ,  CARMONA ALVAREZ, Francisco José ,  CABALLERO JIMENEZ, Pablo

IPC分类号： A23J1/10 ,  A23J3/04 ,  A23K10/26 ,  A61K8/65 ,  C12N1/20

CPC分类号： A23J1/10 ,  A23J3/04 ,  A61K8/65 ,  A23K10/26 ,  A23K20/142 ,  A23K20/147 ,  C12N1/205 ,  C12P21/06 ,  A23J3/341 ,  A61K2800/1020130101 ,  A61Q19/00

公开(公告)号：EP3406625A1

公开(公告)日：2018-11-28

申请号：EP17740952.1

申请日：2017-01-18

申请人： Sinphar Tian-Li (Hangzhou) Pharmacy Company Limited

发明人： WANG, Zhaori ,  LIU, Mingchuan ,  YANG, Shengjie ,  HONG, Da ,  YANG, Jinping

IPC分类号： C07K2/00 ,  C07K1/34 ,  C07K1/14 ,  C12P21/06 ,  A61K38/02 ,  A61K8/64 ,  A23L33/185 ,  A61P25/28 ,  A61P37/02 ,  A61P39/06

CPC分类号： A23L33/185 ,  A61K8/64 ,  A61K38/02 ,  C07K1/14 ,  C07K1/34 ,  C07K2/00 ,  C12P21/06

摘要： The present invention provides a walnut oligopeptide powder, and a preparation method and application thereof. The oligopeptide powder has a peptide content of 80 wt% or higher, wherein 95% or more of the peptides have a molecular weight of less than 1,500 Da. The method comprises extracting walnut proteins using a highly efficient countercurrent extraction process, and subjecting the extract to filtration, enzymolysis, purification with a microfiltration membrane and an ultrafiltration membrane, concentrating, and spray drying to obtain the oligopeptide powder. The oligopeptide powder has an anti-oxidant activity and provides a protective effect for a neuron, and thus can ameliorate or treat memory deterioration and relieve fatigue.

公开(公告)号：EP3256597B1

公开(公告)日：2018-11-14

申请号：EP16703769.6

申请日：2016-02-09

申请人： Sandoz AG

发明人： KELLER, Sascha ,  JASIAK, Anna Justyna ,  FUNKE, René

IPC分类号： C12P21/00 ,  C12P21/02 ,  C12P21/06 ,  C12N9/50

CPC分类号： C12N9/506 ,  C12P21/02 ,  C12P21/06

摘要： Disclosed is a method for producing a recombinant protein of interest, characterised in by the following steps: (a) providing a fusion protein comprising an Npro autoprotease moiety and a protein of interest moiety in inclusion bodies, (b) solubilising the fusion protein in the inclusion bodies by subjecting the inclusion bodies to a solubilisation buffer containing a detergent and wherein the solubilisation buffer contains no chaotropes or chaotropes in a concentration of less than 1.5 M urea (c) refolding the solubilised fusion protein and (d) allowing the fusion protein to be cleaved by the Npro autoprotease moiety under kosmotropic conditions, wherein the recombinant protein of interest is cleaved from the fusion protein, and (e) recovering the protein of interest.

公开(公告)号：EP3027746B1

公开(公告)日：2018-10-17

申请号：EP14831663.1

申请日：2014-07-17

申请人： Academia Sinica

发明人： HO, Meng-Chiao ,  WU, Shih-Hsiung ,  WU, Wan-Ling

IPC分类号： C12N9/50

CPC分类号： C12N9/52 ,  C07K14/39 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/50 ,  C12N15/52 ,  C12P21/06 ,  C12Y304/21

摘要： A fusion gene encoding M. taiwanensis WR-220 keratinase is disclosed. The fusion comprises: (a) a first DNA sequence encoding a protein secretion signal peptide, located at the N-terminus of the fusion gene; (b) a second DNA sequence encoding an inhibitory domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the first DNA sequence; and (c) a third DNA sequence encoding a catalytic domain of M. taiwanensis WR-220 keratinase, linked in translation frame with the second DNA sequence, wherein the fusion gene is a non-naturally occurring chimeric DNA. Also disclosed are a method for preparation of the catalytic domain of M. taiwanensis WR-220 keratinase, and use of the M. taiwanensis WR-220 keratinase.

公开(公告)号：EP2134853B1

公开(公告)日：2018-07-18

申请号：EP08776351.2

申请日：2008-04-03

申请人： Oxyrane UK Limited ,  Universiteit Gent ,  VIB vzw

发明人： CALLEWAERT, Nico, Luc, Marc ,  VERVECKEN, Wouter ,  MARCEL DE POURCQ, Karen, Jacqueline ,  GEYSENS, Steven, Christian, Jozef ,  GUERFAL, Mouna

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24 ,  C07K14/39

CPC分类号： C12N9/2488 ,  A61K38/00 ,  C07K14/00 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/005 ,  C12P21/06 ,  C12Y204/01232 ,  C12Y302/01024 ,  C12Y302/01045 ,  C12Y302/01046 ,  C12Y302/01084 ,  C12Y302/01113 ,  Y02P20/52

摘要： The present invention provides means and methods for enhancing the production of membrane or secreted proteins in genetically engineered cells. In particular, the invention provides nucleic acid molecules encoding HAC1 proteins and uses thereof.

公开(公告)号：EP3311175A1

公开(公告)日：2018-04-25

申请号：EP16751148.4

申请日：2016-06-16

申请人： Novak, Petr ,  Volny, Michael ,  Pompach, Petr ,  Ruzicka, Viktor

发明人： Novak, Petr ,  Volny, Michael ,  Pompach, Petr ,  Ruzicka, Viktor

IPC分类号： G01N33/68 ,  G01N1/40

CPC分类号： G01N33/6851 ,  C07K14/4717 ,  C12P21/06 ,  G01N33/6848 ,  G01N33/6893 ,  G01N2333/4713 ,  G01N2560/00 ,  H01J49/0418

摘要： The invention relates to the affinity plate for haptoglobin phenotype determination, the kit comprising it, and the method of haptoglobin phenotype determination by detection of its a subunits by means of mass spectrometry desorption ionization techniques after preceding preconcentration of haptoglobin on the surface of the affinity plate with immobilized anti-haptoglobin antibody that was deposited onto this surface from gas phase after preceding electrospray ionization and heat desolvation. Then the signals of mass spectra corresponding to α1 and/or α2 subunits can be found and thus determined the haptoglobin phenotype, whereas only al subunit exists in phenotype 1-1, both the subunits a1 and α2 exist in phenotype 2-1, and only α2 subunit exists in phenotype

公开(公告)号：EP3138576A4

公开(公告)日：2017-11-29

申请号：EP15785722

申请日：2015-04-30

申请人： UNIV KINKI

发明人： MORIMOTO KOICHI ,  KUNII SAORI ,  YAMAMOTO EI ,  ITO HIROYUKI ,  KUBOKI YOSHINORI

IPC分类号： A61K38/17 ,  A23J3/06 ,  A23J3/34 ,  A61P43/00 ,  C07K14/78 ,  C12P21/06

CPC分类号： C07K14/78 ,  A23J3/06 ,  A23J3/34 ,  A61K38/17 ,  C12P21/06

摘要： To provide a differentiation-inducing composition and a method for producing the differentiation-inducing composition, the present disclosure describes use of a degradation product containing at least a portion of the triple helical domain of collagen or atelocollagen.

公开(公告)号：EP2508612B1

公开(公告)日：2017-11-29

申请号：EP12000268.8

申请日：2008-04-03

申请人： Oxyrane UK Limited ,  Universiteit Gent ,  VIB vzw

发明人： Callewaert, Nico Luc Marc ,  Vervecken, Wouter ,  de Pourq, Karen Jacqueline Marcel ,  Geysens, Steven Christian Jozef ,  Guerfal, Mouna

IPC分类号： C12P21/00 ,  C12N9/10 ,  C12N9/24 ,  C07K14/39

CPC分类号： C12N9/2488 ,  A61K38/00 ,  C07K14/00 ,  C12N9/1051 ,  C12N9/24 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/005 ,  C12P21/06 ,  C12Y204/01232 ,  C12Y302/01024 ,  C12Y302/01045 ,  C12Y302/01046 ,  C12Y302/01084 ,  C12Y302/01113 ,  Y02P20/52

摘要： The present invention provides means and methods for enhancing the production of membrane or secreted proteins in genetically engineered cells. In particular, the invention provides nucleic acid molecules encoding HAC1 proteins and uses thereof.

公开(公告)号：EP2728011B1

公开(公告)日：2017-10-25

申请号：EP11842461.3

申请日：2011-07-27

申请人： Queizuar, S.L.

发明人： PEREIRA RAMOS, Benigno ,  PEREIRA RODRIGUEZ, Angel ,  ESTEVEZ TELLE, Natalia ,  FUCIÑOS GONZALEZ, Juan Pablo ,  RUA RODRIGUEZ, Maria Luisa ,  PASTRANA CASTRO, Lorenzo

IPC分类号： C12P21/06 ,  A23J3/34

CPC分类号： A23J3/343 ,  A23J1/205 ,  A23L33/18 ,  C07K4/12 ,  C12P21/06