公开(公告)号：EP1313873B1

公开(公告)日：2008-03-26

申请号：EP01956637.1

申请日：2001-07-24

申请人： Aventis Pharma S.A.

发明人： DUMAS, Bruno ,  CAUET, Gilles ,  ACHSTETTER, Tilman ,  DEGRYSE, Eric

IPC分类号： C12N1/16 ,  C12N15/53 ,  C12N9/02 ,  C12P33/06

CPC分类号： C12N9/0087 ,  C12P33/06

摘要： The invention concerns novel yeast strains, methods and genetic constructs for preparing them, and their use for synthesising or modifying steroid compounds. More particularly, the invention concerns strains having reduced 20α-HSD activity, in particular by modification of the GCY1 and/or YPR1 genes. The inventive yeast strains enable to improve the efficacy of synthesis or to increase selectivity or yields of the process, as well as the quality of the final product. The inventive strains, methods and compounds are useful for researching, developing and producing products with therapeutic or prophylactic activity, in a human or an animal, in particular steroid derivatives.

公开(公告)号：EP1385980B1

公开(公告)日：2010-10-27

申请号：EP02708406.0

申请日：2002-01-29

申请人： Aventis Pharma S.A.

发明人： SPAGNOLI, Roberto ,  ACHSTETTER, Tilman ,  CAUET, Gilles ,  DEGRYSE, Eric ,  DUMAS, Bruno ,  POMPON, Denis ,  WINTER, Jacques

IPC分类号： C12P33/00 ,  C12P33/08 ,  C12P33/18

CPC分类号： C12Y114/15004 ,  C12P33/005 ,  C12P33/08 ,  C12P33/18 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y114/9901 ,  C12Y118/01002

摘要： The invention concerns genetically modified yeast strains autonomously producing steroids from a simple carbon source. The invention also concerns a method for producing steroids from said yeast strains.

公开(公告)号：EP0977868B1

公开(公告)日：2004-07-21

申请号：EP99901708.0

申请日：1999-02-04

申请人： Aventis Pharma S.A.

发明人： ACHSTETTER, Tilman ,  CAUET, Gilles ,  DEGRYSE, Eric

IPC分类号： C12N15/54 ,  C12N1/19 ,  C12P33/00 ,  C12N9/10

CPC分类号： C12N9/1029 ,  C12P33/00

摘要： The invention concerns a modified yeast strain wherein the acetyl-CoA pregnenolone acetyl-transferase activity (APAT) is eliminated by alteration of the gene coding for said activity and which results in stabilisation of the 3 beta -hydroxysteroids.

公开(公告)号：EP1385980A2

公开(公告)日：2004-02-04

申请号：EP02708406.0

申请日：2002-01-29

申请人： Aventis Pharma S.A.

发明人： SPAGNOLI, Roberto ,  ACHSTETTER, Tilman ,  CAUET, Gilles ,  DEGRYSE, Eric ,  DUMAS, Bruno ,  POMPON, Denis ,  WINTER, Jacques

IPC分类号： C12P33/00 ,  C12P33/08 ,  C12P33/18 ,  C12N1/19 ,  A61K35/72

CPC分类号： C12Y114/15004 ,  C12P33/005 ,  C12P33/08 ,  C12P33/18 ,  C12Y114/15006 ,  C12Y114/99009 ,  C12Y114/9901 ,  C12Y118/01002

摘要： The invention concerns genetically modified yeast strains autonomously producing steroids from a simple carbon source. The invention also concerns a method for producing steroids from said yeast strains.

公开(公告)号：EP1313873A1

公开(公告)日：2003-05-28

申请号：EP01956637.1

申请日：2001-07-24

申请人： Aventis Pharma S.A.

发明人： DUMAS, Bruno ,  CAUET, Gilles ,  ACHSTETTER, Tilman ,  DEGRYSE, Eric

IPC分类号： C12P33/06 ,  C12N15/53 ,  C12N1/16

CPC分类号： C12N9/0087 ,  C12P33/06

摘要： The invention concerns novel yeast strains, methods and genetic constructs for preparing them, and their use for synthesising or modifying steroid compounds. More particularly, the invention concerns strains having reduced 20α-HSD activity, in particular by modification of the GCY1 and/or YPR1 genes. The inventive yeast strains enable to improve the efficacy of synthesis or to increase selectivity or yields of the process, as well as the quality of the final product. The inventive strains, methods and compounds are useful for researching, developing and producing products with therapeutic or prophylactic activity, in a human or an animal, in particular steroid derivatives.