公开(公告)号：EP2225382B1

公开(公告)日：2016-11-09

申请号：EP08855448.0

申请日：2008-11-28

申请人： BASF SE

发明人： HAUER, Bernhard ,  SCHNEIDER, Nina ,  OKRASA, Krzysztof ,  MICKLEFIELD, Jason ,  LEYS, David ,  LEVY, Colin

IPC分类号： C12P7/40 ,  C12P7/42 ,  C12N9/88 ,  C12N9/90 ,  C12N15/61

CPC分类号： C12N9/88 ,  C07K14/235 ,  C07K2299/00 ,  C12N9/90 ,  C12P7/40 ,  C12P7/42 ,  C12Q1/00 ,  C12Y401/01075 ,  C12Y401/01076

公开(公告)号：EP2225382A2

公开(公告)日：2010-09-08

申请号：EP08855448.0

申请日：2008-11-28

申请人： BASF SE

发明人： HAUER, Bernhard ,  SCHNEIDER, Nina ,  OKRASA, Krzysztof ,  MICKLEFIELD, Jason ,  LEYS, David ,  LEVY, Colin

IPC分类号： C12P7/40 ,  C12P7/42 ,  C12N9/88 ,  C12N9/90 ,  C12N15/61 ,  C12Q1/00

CPC分类号： C12N9/88 ,  C07K14/235 ,  C07K2299/00 ,  C12N9/90 ,  C12P7/40 ,  C12P7/42 ,  C12Q1/00 ,  C12Y401/01075 ,  C12Y401/01076

摘要： The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.