公开(公告)号：EP3074754A4

公开(公告)日：2017-07-26

申请号：EP14861862

申请日：2014-11-10

Applicant: BECTON DICKINSON CO

Inventor： BORNHEIMER SCOTT ,  GOLDBERG EDWARD

IPC: G01N21/25 ,  G01J3/02 ,  G01J3/04 ,  G01J3/42 ,  G01N21/03 ,  G01N21/31 ,  G01N21/359 ,  G01N21/59 ,  G01N21/64 ,  G01N33/72 ,  G01N35/00

CPC classification number: G01N33/721 ,  G01J3/0208 ,  G01J3/04 ,  G01J3/42 ,  G01N21/31 ,  G01N21/359 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2021/0346 ,  G01N2021/6439 ,  G01N2021/6491 ,  G01N2201/062

Abstract: Aspects of the present disclosure include methods and systems for assaying a sample for an analyte. Methods according to certain embodiments include illuminating a sample with a slit-shaped beam of light, detecting light transmitted through the sample, determining absorbance of the transmitted light at one or more wavelengths and calculating concentration of the analyte based on the absorbance to assay the sample for the analyte. Systems for practicing the subject methods are also described.

Abstract translation: 本公开的方面包括用于分析样品以获得分析物的方法和系统。 根据某些实施方案的方法包括用狭缝状光束照射样品，检测透过样品的光，确定透射光在一个或多个波长处的吸光度，并基于吸光度计算分析物的浓度以测定样品 为分析物。 还描述了用于实践主题方法的系统。

公开(公告)号：EP2753924A4

公开(公告)日：2015-04-22

申请号：EP12830820

申请日：2012-09-06

Applicant: BECTON DICKINSON CO

Inventor： KIRAKOSSIAN HRAIR ,  GOLDBERG EDWARD ,  RECKTENWALD DIETHER

IPC: G01N33/574 ,  G01N15/14

CPC classification number: G01N33/56966 ,  G01N15/1459 ,  G01N33/533 ,  G01N33/56972 ,  G01N33/57484 ,  G01N33/57492 ,  G01N33/57496 ,  G01N2015/1402 ,  G01N2015/1477

Abstract: The present disclosure provides cytometric methods for the detection of rare target cells in a sample. In certain aspects, the methods and compositions may facilitate the detection of rare target cells, such as circulating tumor cells (CTCs), in a biological sample such as blood. Aspects of the methods include contacting the sample with first and second binding members that specifically bind to a marker of the rare target cell, and cytometrically assaying the sample for the presence of cells comprising bound first and second binding members to detect the rare target cell in the sample. Also provided are systems, compositions, and kits for practicing the subject methods.

Abstract translation: 本发明提供一种用于检测样品中的罕见的靶细胞的流式细胞术方法。 在某些方面，该方法和组合物可以促进稀有靶细胞的检测，循环肿瘤细胞：生物样品中的如（的CTC）：诸如血液。 的方法的各方面包括使与第一样品和第二结合成员并特异性地结合至该稀少目标细胞的标志物，并进行细胞计数测定为细胞的存在的样品，其包括结合第一和​​第二结合成员来检测稀少目标细胞中 样品。 因此，提供了系统，组合物和用于实施本发明方法的试剂盒。