公开(公告)号：EP4458962A1

公开(公告)日：2024-11-06

申请号：EP21969710.9

申请日：2021-12-31

申请人： BGI Shenzhen

发明人： WANG, Lele ,  ZENG, Tao ,  LIU, Zhenjun ,  CHEN, Junyi ,  ZHAO, Ziyu ,  LI, Denghui ,  GUO, Fei ,  JI, Zhouxiang ,  WANG, Ou ,  LI, Yuxiang ,  DONG, Yuliang ,  ZHANG, Wenwei ,  XU, Xun

IPC分类号： C12N9/14 ,  C12N15/55 ,  C12M1/34 ,  C12Q1/6869 ,  G01N33/48

摘要： The present invention provides a helicase BCH2X, comprising an amino acid sequence represented by any one of SEQ ID NOs: 1-3. The present invention also provides a complex structure comprising the helicase BCH2X and a binding moiety for binding polynucleotides. The present invention also provides a use of the helicase BCH2X or the complex structure comprising same in the control and characterization of polynucleotides and single-molecule nanopore sequencing.

公开(公告)号：EP4458987A1

公开(公告)日：2024-11-06

申请号：EP21969698.6

申请日：2021-12-31

申请人： BGI Shenzhen

发明人： XU, Xun ,  GUO, Fei ,  HU, Qiaoxia ,  LI, Denghui ,  CHEN, Junyi ,  WANG, Lele ,  ZHAO, Ziyu ,  JI, Zhouxiang ,  ZENG, Tao ,  WANG, Ou ,  LI, Yuxiang ,  DONG, Yuliang ,  ZHANG, Wenwei

IPC分类号： C12Q1/6869 ,  C12N9/12 ,  G01N27/447

摘要： Provided is helicase BCH1X, which comprises an amino acid sequence represented by SEQ ID NO: 1 or 2. Further provided are a complex structure comprising helicase BCH1X and a binding moiety used for binding to a polynucleotide, and a use thereof in the control and characterization of a polynucleotide and in single molecule nanopore sequencing.

公开(公告)号：EP4435098A1

公开(公告)日：2024-09-25

申请号：EP21963712.1

申请日：2021-11-15

申请人： BGI Shenzhen

发明人： AN, Qun ,  LIN, Qibin ,  GUO, Fei ,  XIE, Qingqing ,  ZHENG, Yue ,  XI, Feng ,  DONG, Yuliang ,  ZHANG, Wenwei

IPC分类号： C12N9/12 ,  C12N15/54 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12N9/10 ,  C12N9/12

摘要： Provided is a chimeric DNA polymerase, comprising: a first peptide segment, which has at least 80% homology with at least part of the amino acid sequence in the N-terminal domain of a 90N DNA polymerase; a second peptide segment, which has at least 80% homology with at least part of the amino acid sequence in the exonucleolytic domain of a KOD DNA polymerase; a third peptide segment, which has at least 80% homology with at least some of the amino acids in the N-terminal domain of the 90N DNA polymerase; a fourth peptide segment, which has at least 80% homology with at least some of the amino acids in the palm domain of a KOD DNA polymerase; a fifth peptide segment, which has at least 80% homology with at least some of the amino acids in the finger domain of the Pfu DNA polymerase; a sixth peptide segment, which has at least 80% homology with at least some of the amino acids in the palm domain of the KOD DNA polymerase; and a seventh peptide segment, which has at least 80% homology with at least some of the amino acids in the thumb domain of the 90N DNA polymerase.