公开(公告)号：EP3854872A1

公开(公告)日：2021-07-28

申请号：EP18936847.5

申请日：2018-10-11

申请人： BGI Shenzhen

发明人： ZHANG, Zhougang ,  LIU, Huanhuan ,  ZHENG, Yue ,  ZHOU, Yujun ,  XIA, Jun ,  DONG, Yuliang ,  XU, Chongjun ,  ZHANG, Wenwei

IPC分类号： C12N9/12 ,  C12N15/52 ,  C12N15/63

摘要： Provided are a Phi29 DNA polymerase mutant with improved thermal stability and an use thereof in sequencing. The phi29 DNA polymerase mutant is represented by A) or B) below: the DNA polymerase mutant represented by the A) is a protein having DNA polymerase activity that is obtained by modifying at least one amino acid residue(s) in the following six sites in a phi29 DNA polymerase amino acid sequence: the 97-th, 123-th, 217-th, 224-th, 515-th, and 474-th sites; the DNA polymerase mutant represented by the B) is a protein having DNA polymerase activity that is derived from the A) by adding a tag sequence to a terminal of the amino acid sequence of the protein represented by the A).