公开(公告)号：EP4442802A1

公开(公告)日：2024-10-09

申请号：EP21965926.5

申请日：2021-11-30

申请人： BGI Shenzhen

发明人： ZHOU, Fan ,  XU, Xun ,  NI, Jielei ,  SHEN, Mengzhe ,  WANG, Wenbing

IPC分类号： C12M1/16 ,  C12M1/00 ,  G01N21/65

CPC分类号： C12M1/16 ,  G01N21/65 ,  C12M1/00

摘要： The present application provides a detection chip and a preparation method therefor. The detection chip comprises a substrate material layer; a feature array comprising a plurality of feature units distributed on the substrate material layer; and a feature array protection structure formed on the base material layer, the feature array being distributed in the feature array protection structure, wherein the feature array protection structure comprises at least an adsorption material for adsorbing a biological sample. According to the present application, the excitation power enhanced by the detection chip is improved by at least a plurality of orders of magnitude compared with the incident laser power, and with the design optimization and process improvement of the feature array, the enhancement proportion can be further improved, such that the excitation power received by the fluorescent dye is improved without changing the power of the laser, and stronger fluorescence signal intensity is generated. Therefore, the exposure time can be effectively shortened in a case that the imaging quality and the laser power are not changed, and the sequencing flux is improved.

公开(公告)号：EP4024031A1

公开(公告)日：2022-07-06

申请号：EP20924340.1

申请日：2020-03-09

申请人： BGI Shenzhen

发明人： NI, Jielei ,  NI, Ming ,  ZHOU, Fan ,  SU, Zeyu ,  JI, Ke ,  WEI, Dong ,  SHEN, Mengzhe ,  LIANG, Yuanqing ,  LI, Mei ,  XU, Xun

IPC分类号： G01N21/64

摘要： Disclosed are a super-resolution imaging system (1, 41, 51), a super-resolution imaging method, a biological sample identification system (4, 61) and method, a nucleic acid sequencing imaging system (5) and method, and a nucleic acid identification system (6) and method. The super-resolution imaging system (1, 41, 51) comprises an illumination system (A) and an imaging system (B). The illumination system (A) outputs excitation light to irradiate a biological sample to generate excited light, and the imaging system (B) collects and records the excited light to generate an excited light image. The illumination system (A) comprises an excitation light source (10, 10a) and a structured light generation and modulation device (11, 11a). The excitation light source (10, 10a) outputs the excitation light, and the structured light generation and modulation device (11, 11a) modulates the excitation light into structured light to irradiate the biological sample to generate the excited light. The structured light generation and modulation device (11, 11a) comprises a structured light control device (113, 113a). The structured light control device (113, 113a) controls the structured light generation and modulation device (11, 11a) to change the phase of the structured light output by the structured light generation and modulation device and the direction of an illumination pattern of the structured light projected on the biological sample. The imaging system (B) photographs images of excited light excited by excitation light of at least a wavelength in at least an imaging field of view, in conjunction with the change in the phase of the structured light and/or the direction of the illumination pattern, thus improving the imaging efficiency.