-
1.发明公开Growth hormone analogs, pharmaceutical and veterinary compositions containing said analogs and methods for production thereof 失效生长激素类似物，药物和含有它们和它们的制备方法兽药组合物。
公开(公告)号：EP0319049A2
公开(公告)日：1989-06-07
申请号：EP88121246.8
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
摘要： Disclosed are bGH and hGH analogs having the activity of the respective naturally occurring substances and a similar amino acid sequence varying from the sequence of the respective naturally occurring substances. Further there are disclosed pharmaceutical and veterinary compositions containing said analogs as well as methods for producing and purifying said analogs.
摘要翻译： 公开的是具有与天然存在的物质respectivement的活性和相似的氨基酸序列与天然存在的物质respectivement的序列不同的bGH和hGH类似物。 进一步有游离缺失盘药物和含有所述类似物以及方法生产和纯化兽用组合物，所述类似物。
-
2.发明公开Method for recovering purified growth hormones from genetically engineered microorganisms 失效一种用于从遗传修饰的微生物纯化生长激素的恢复过程。
公开(公告)号：EP0173215A2
公开(公告)日：1986-03-05
申请号：EP85110371.3
申请日：1985-08-19
IPC分类号： C12P21/02
CPC分类号： C07K14/61
摘要： 57 This invention concerns a method for recovering a purified eucaryotic growth hormone or an analog thereof from a bacterial cell in which the growth hormone or analog has been produced by means of expression of a DNA sequence encoding the hormone or analog. The method involves the following steps: (a) disrupting the cell wall of the bacterial cell or fragments thereof to produce a lysate; (b) separating a precipitate containing particulate and insoluble material from the lysate; (c) preparing suspension of the separated precipitate in an appropriate buffer solution; (d) treating the suspension to effect contact between liquid and solids; (e) separating partially or completely precipitated material from the liquid-solids contacted suspension; (f) solubilizing the separated precipitate in a sufficient amount of water and adjusting the pH to a suitable alkaline pH to form a solution; (g) separating the solubilized hormone or analog from other cellular components by ultrafiltration under suitable conditions to produce an ultrafiltrate containing the hormone or analog and a retentate, and (h) treating the solubilized hormone or analog so as to concentrate the hormone or analog and thereby recover purified hormone or analog.
Separations may be effected by ultrafiltration or centrifugation. Sonication as well as the ultrafiltration in step (i) may be repeated. Additionally, most of the process steps may be performed in a continuous flow and may be automated.
Suitable hormone-producing bacterial cells for use in this invention include genetically modified strains of Escherichia coli. Growth hormones which can be recovered and purified using this invention include bovine, porcine, chicken, and human growth hormones, and analogs thereof.摘要翻译： 本发明涉及用于回收纯化的真核生长激素或其类似物以从其中生长激素或类似物已经通过编码激素或类似物的DNA序列的表达的方式生产的细菌细胞的方法。 该方法涉及以下步骤：（a）破坏细菌细胞或其片段的细胞壁以产生裂解物; （B）将含有从裂解物微粒和不溶性物质的沉淀物; （C）制备在适当的缓冲溶液中分离的沉淀物的悬浮液; （D）处理该悬浮液，以实现液体和固体之间的接触; （E）中分离部分或完全从液体 - 固体接触悬浮呼叫沉淀材料; （F）溶解在水足量的分离的沉淀物并调节pH至合适的碱性pH以形成溶液; （G）通过在合适的条件下超滤分离与其他细胞组分溶解的激素或类似物以产生超滤液通过含有激素或类似物和滞留物，和（h）处理该增溶的激素或类似物，以便集中激素或类似物和 从而回收纯化的激素或类似物。 分离可以通过超滤或离心来进行。 超声处理，以及在步骤（i）的超滤可以被重复。 此外，大多数的过程步骤可以在连续流执行，并且可以被自动化。 合适的激素产生的细菌细胞用于本发明用途包括遗传修饰的大肠杆菌菌株。 可以回收和利用本发明纯化生长激素包括牛，猪，鸡，和人生长激素，及其类似物。
-
3.发明授权Method for recovering purified growth hormones from genetically engineered microorganisms 失效从遗传工程微生物中回收纯化生长激素的方法
-
4.发明公开Growth hormone analogs, pharmaceutical and veterinary compositions containing said analogs and methods for production thereof 失效含有该类似物的生长激素类似物，药物和兽医学组合物及其生产方法
公开(公告)号：EP0319049A3
公开(公告)日：1990-01-03
申请号：EP88121246.8
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
摘要： Disclosed are bGH and hGH analogs having the activity of the respective naturally occurring substances and a similar amino acid sequence varying from the sequence of the respective naturally occurring substances. Further there are disclosed pharmaceutical and veterinary compositions containing said analogs as well as methods for producing and purifying said analogs.
-
5.发明公开Process for the preparation of superoxide dismutase by recombinant DNA-technology 失效Verfahren zur Herstellung von Superoxid dismutase durch rekombinante DNS Technologie
公开(公告)号：EP0691406A1
公开(公告)日：1996-01-10
申请号：EP95114069.8
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
CPC分类号： C12N9/0089 , A61K38/00 , C07K14/61 , C12N15/73 , Y10S530/82 , Y10S530/825 , Y10S930/12
摘要： Disclosed is an improved plasmid which upon introduction into a suitable Escherichia coli host cell containing the thermolabile repressor C I , renders the host cell capable, upon increasing the temperature of the host cell to a temerature at which the repressor is destroyed, of effecting expression of a gene encoding superoxide dismutase inserted into the vector, and production of the superoxide dismutase encoded by the gene.
摘要翻译： 公开了一种改进的质粒，其在引入含有不耐热阻遏物CI的合适的大肠杆菌宿主细胞中时，使宿主细胞在宿主细胞的温度升高至阻遏物被破坏的温度时能够发挥作用 将编码超氧化物歧化酶的基因插入载体，并产生由该基因编码的超氧化物歧化酶。
-
6.发明公开Expression vectors for enhanced production of polypeptides, plasmids containing the vectors, hosts containing the plasmids, products manufactured thereby and related methods 失效用于产生多肽的增加的表达载体，含有这些质粒的载体，通过载体含有这些质粒的宿主产生的产物和相应的方法。
公开(公告)号：EP0131843A1
公开(公告)日：1985-01-23
申请号：EP84107717.5
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
CPC分类号： C12N9/0089 , A61K38/00 , C07K14/61 , C12N15/73 , Y10S530/82 , Y10S530/825 , Y10S930/12
摘要： @ An improved vector upon introduction into a suitable bacterial host containing the thermolabile repressor Ct renders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene inserted into the vector and production of polypeptide encoded by the gene. The vector is a double-stranded DNA molecule which includes In 5' to 3' order the following: a DNA sequence which contains the promoter and operator PLOL from lambda bacteriophage; the N utilization site for binding ant i terminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; and ATG initiation codon or a DNA sequence which is converted Into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation oodon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable trait which is manifested when the vector is present in the host cell.
-
7.发明授权Growth hormone analogs, pharmaceutical and veterinary compositions containing said analogs and methods for production thereof 失效生长激素类似物，药学和兽医学组合物含有它们和它们的制备方法
公开(公告)号：EP0319049B1
公开(公告)日：1996-04-17
申请号：EP88121246.8
申请日：1984-07-03
-
8.发明授权Expression vectors for enhanced production of polypeptides, plasmids containing the vectors, hosts containing the plasmids, products manufactured thereby and related methods 失效用于增强多肽生物的表达载体，含有载体的PLASMIDS，含有PLASMIDS的载体，其制造的产品及相关方法
公开(公告)号：EP0131843B1
公开(公告)日：1991-09-04
申请号：EP84107717.5
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
CPC分类号： C12N9/0089 , A61K38/00 , C07K14/61 , C12N15/73 , Y10S530/82 , Y10S530/825 , Y10S930/12
-
9.发明公开Method for recovering purified growth hormones from genetically engineered microorganisms 失效从遗传工程微生物中回收纯化生长激素的方法
公开(公告)号：EP0173215A3
公开(公告)日：1987-11-25
申请号：EP85110371
申请日：1985-08-19
CPC分类号： C07K14/61
摘要： 57 This invention concerns a method for recovering a purified eucaryotic growth hormone or an analog thereof from a bacterial cell in which the growth hormone or analog has been produced by means of expression of a DNA sequence encoding the hormone or analog. The method involves the following steps: (a) disrupting the cell wall of the bacterial cell or fragments thereof to produce a lysate; (b) separating a precipitate containing particulate and insoluble material from the lysate; (c) preparing suspension of the separated precipitate in an appropriate buffer solution; (d) treating the suspension to effect contact between liquid and solids; (e) separating partially or completely precipitated material from the liquid-solids contacted suspension; (f) solubilizing the separated precipitate in a sufficient amount of water and adjusting the pH to a suitable alkaline pH to form a solution; (g) separating the solubilized hormone or analog from other cellular components by ultrafiltration under suitable conditions to produce an ultrafiltrate containing the hormone or analog and a retentate, and (h) treating the solubilized hormone or analog so as to concentrate the hormone or analog and thereby recover purified hormone or analog. Separations may be effected by ultrafiltration or centrifugation. Sonication as well as the ultrafiltration in step (i) may be repeated. Additionally, most of the process steps may be performed in a continuous flow and may be automated. Suitable hormone-producing bacterial cells for use in this invention include genetically modified strains of Escherichia coli. Growth hormones which can be recovered and purified using this invention include bovine, porcine, chicken, and human growth hormones, and analogs thereof.
-
-
-
-
-
-
-
-
搜索结果
9 条记录 (耗时 0.012 秒)
