公开(公告)号：EP0576792A2

公开(公告)日：1994-01-05

申请号：EP93105848.1

申请日：1993-04-13

申请人： BIOGAL GYOGYSZERGYAR RT.

发明人： Ott, István, Dr. ,  Klupp, Tibor, Dr. ,  Molnár, István ,  Patthy, András, Dr. ,  Barta, István ,  Berk -T th, Zsuzsa, Ir.-Chem. ,  Ambrus, Gábor, Dr. Ir.-Chem. ,  Salát, János ,  Tegdes, Anik ,  Moravcsik, Imre, Ir.-Chem. ,  Együd, Cecilia ,  Albrecht, Károly ,  Könczöl, Kálmán, Dr. Ir.-Chem. ,  Vincze, Attila ,  Barabás, Eva, Dr. ,  Máté, György, Ir.-Chem. ,  Botond Kiss, György Dr. ,  Kiss, Péter ,  Pölya, Kálmán, Dr. ,  Erdei, Janos Dr. ,  Erdei, Jan s Dr. ,  Zilahi, Erika

IPC分类号： C12N15/15

CPC分类号： C07K14/815 ,  A61K38/00 ,  C07K2319/00 ,  C12N15/76

摘要： The present invention relates to a process for producing recombinant desulphatohirudin by means of culturing microorganisms.
Concerning the codon usage of microorganisms the synthesized nucleotide sequences were joined downstream of and in reading frame with isolated promoters and signal sequences, subsequently the expression/secretion cassettes comprising the foregoing elements were inserted into plasmid DNAs allowing the cultivation of cells under selective culture conditions. E. coli, Saccharomyces and Streptomyces species were transformed with the said recombinant plasmids to biosynthesize the thrombin inhibitor desulphatohirudin HV-1 which was then isolated and identified.
The thus-produced desulphatohirudin can be used to inhibit blood coagulation.

摘要翻译： 本发明涉及通过培养微生物生产重组脱硫水蛭素的方法。 关于微生物的密码子使用，合成的核苷酸序列在分离的启动子和信号序列的下游和阅读框架中连接，随后将包含前述元件的表达/分泌盒插入允许在选择性培养条件下培养细胞的质粒DNA。 用所述重组质粒转化大肠杆菌，酵母属和链霉菌物种以生物合成凝血酶抑制剂脱硫水蛭素HV-1，然后分离和鉴定。 由此产生的脱硫水蛭素可用于抑制血液凝固。

公开(公告)号：EP0576792A3

公开(公告)日：1994-08-24

申请号：EP93105848.1

申请日：1993-04-13

申请人： BIOGAL GYOGYSZERGYAR RT.

发明人： Ott, István, Dr. ,  Klupp, Tibor, Dr. ,  Molnár, István ,  Patthy, András, Dr. ,  Barta, István ,  Berk -T th, Zsuzsa, Ir.-Chem. ,  Ambrus, Gábor, Dr. Ir.-Chem. ,  Salát, János ,  Tegdes, Anik ,  Moravcsik, Imre, Ir.-Chem. ,  Együd, Cecilia ,  Albrecht, Károly ,  Könczöl, Kálmán, Dr. Ir.-Chem. ,  Vincze, Attila ,  Barabás, Eva, Dr. ,  Máté, György, Ir.-Chem. ,  Botond Kiss, György Dr. ,  Kiss, Péter ,  Pölya, Kálmán, Dr. ,  Erdei, Janos Dr. ,  Erdei, Jan s Dr. ,  Zilahi, Erika

IPC分类号： C12N15/15

CPC分类号： C07K14/815 ,  A61K38/00 ,  C07K2319/00 ,  C12N15/76

摘要： The present invention relates to a process for producing recombinant desulphatohirudin by means of culturing microorganisms. Concerning the codon usage of microorganisms the synthesized nucleotide sequences were joined downstream of and in reading frame with isolated promoters and signal sequences, subsequently the expression/secretion cassettes comprising the foregoing elements were inserted into plasmid DNAs allowing the cultivation of cells under selective culture conditions. E. coli, Saccharomyces and Streptomyces species were transformed with the said recombinant plasmids to biosynthesize the thrombin inhibitor desulphatohirudin HV-1 which was then isolated and identified. The thus-produced desulphatohirudin can be used to inhibit blood coagulation.

摘要翻译： 本发明涉及通过培养微生物来生产重组脱硫水蛭素的方法。 关于微生物的密码子使用，合成的核苷酸序列连接在分离的启动子和信号序列的下游并在阅读框中，随后将包含上述元件的表达/分泌盒插入质粒DNA中，允许在选择性培养条件下培养细胞。 用所述重组质粒转化大肠杆菌，酵母属和链霉菌属物种以生物合成凝血酶抑制剂脱硫水蛭素HV-1，然后分离并鉴定凝血酶抑制剂脱硫水蛭素HV-1。 由此产生的脱硫水蛭素可用于抑制血液凝固。