专利检索 ap:("BIOGEN, INC.") AND inv:"ERNST, Joachim, F." 第 1 页

1.

发明授权
ENHANCED SECRETION OF HETEROLOGOUS PROTEINS BY HOSTS USING SUBSTITUTED PROMOTERS 失效
标题翻译： INCREASED异种蛋白质由宿主细胞的沉淀 - 使用取代推动者。

公开(公告)号：EP0283475B1

公开(公告)日：1994-05-18

申请号：EP86906826.2

申请日：1986-11-24

申请人： BIOGEN, INC.

发明人： ERNST, Joachim, F. , SCHMEISSNER, Ursula

IPC分类号： C12N15/81 , C12N1/19 , C12N15/68

CPC分类号： C07K14/65 , C07K14/395 , C07K2319/02 , C12N15/69 , C12N15/81

摘要： Improved secretion of heterologous proteins by hosts such as yeast by using promoters of at most intermediate strength with heterologous DNA secretion signal sequences. A promoter of at most intermediate strength, such as the actin (ACT) or iso-l-cytochrome c (CYCl) promoter in S. cerevisiae is operatively linked to a DNA signal sequence, such as the MFalpha) signal sequence. A DNA sequence for a selected protein, such as somatomedin C (SMC), tissue plasminogen activator (TPA) or tumor necrosis factor (TNF), may be operatively linked to the DNA signal sequence.