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公开(公告)号：EP0698118A1
公开(公告)日：1996-02-28
申请号：EP95913592.0
申请日：1995-03-07
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
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公开(公告)号：EP0607422B1
公开(公告)日：2002-03-13
申请号：EP93919972.5
申请日：1993-08-12
发明人： SHI, Shan-Rong , TANDON, Atul, K. , KALRA, Krishan, L. , MALHOTRA, Nagesh , SU, Sheng-Hui , YU, Cheng-Zhi
IPC分类号： G01N33/53 , G01N33/543 , G01N1/30
CPC分类号： G01N1/30 , G01N33/5306
摘要： A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed with an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
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公开(公告)号：EP0698118B1
公开(公告)日：2003-12-03
申请号：EP95913592.2
申请日：1995-03-07
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
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公开(公告)号：EP0607422A1
公开(公告)日：1994-07-27
申请号：EP93919972.0
申请日：1993-08-12
发明人： SHI, Shan-Rong , TANDON, Atul, K. , KALRA, Krishan, L. , MALHOTRA, Nagesh , SU, Sheng-Hui , YU, Cheng-Zhi
CPC分类号： G01N1/30 , G01N33/5306
摘要： L'invention se rapporte à un procédé visant à rétablir l'immunoréactivité d'un tissu, notamment d'un tissu décalcifié, fixé par un agent fixateur aldéhyde et incorporé dans un liquide d'inclusion comprenant généralement de la celloïdine. Le procédé consiste à mettre en contact le tissu avec une solution d'un réactif libérant de l'aldéhyde comprenant un solvant et un réactif libérant de l'aldéhyde, à retirer l'aldéhyde libéré par le réactif libérant l'aldéhyde du contact avec le tissu, à faire réagir l'aldéhyde de manière pratiquement irréversible pour former un dérivé non-aldéhyde, et à retirer la base excédentaire du tissu. Une solution préférée d'un tissu décalcifié incorporé dans la celloïdine comprend de la soude caustique méthanolique au tiers environ du niveau de saturation.
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