专利检索 ap:("BIONEBRASKA, INC.") AND inv:"PARTRIDGE, Bruce E." 第 1 页

1.

发明公开
PRODUCTION OF PEPTIDES USING RECOMBINANT FUSION PROTEIN CONSTRUCTS 失效
标题翻译：生产肽MITTLES重组融合蛋白结构的

公开(公告)号：EP0796335A1

公开(公告)日：1997-09-24

申请号：EP95942995.0

申请日：1995-12-07

申请人： BIONEBRASKA, INC.

发明人： PARTRIDGE, Bruce E. , STOUT, Jay S. , HENRIKSEN, Dennis B. , MANNING, Shane D. , DE LA MOTTE, Rebecca S. , HOLMQUIST, Barton , WAGNER, Fred W.

IPC分类号： C12N15 , C07K1 , C07K5 , C07K7 , C07K14 , C07K19 , C12N1 , C12N9 , C12P21 , C12R1

CPC分类号： C12N9/88 , C07K1/1136 , C07K1/30 , C07K14/60 , C07K14/605 , C07K14/635 , C07K2319/00

摘要： A method for the isolation and/or purification of a recombinant peptide by employing a fusion protein construct which includes a carbonic anhydrase and a variable fused polypeptide is provided. The method includes precipitating either the fusion protein construct or a fragment of the fusion protein construct including the carbonic anhydrase. Inclusion bodies which includes the fusion protein construct and a method of producing a peptide which includes expressing the fusion protein construct as a part of an inclusion body are also provided. Fusion protein constructs which include a carbonic anhydrase and certain target peptides are also provided.