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33 条记录 (耗时 0.015 秒)

    RECOMBINANT PREPARATION OF CALCITONIN FRAGMENTS AND USE THEREOF IN THE PREPARATION OF CALCITONIN AND RELATED ANALOGS
    1.
    发明公开
    RECOMBINANT PREPARATION OF CALCITONIN FRAGMENTS AND USE THEREOF IN THE PREPARATION OF CALCITONIN AND RELATED ANALOGS 失效
    降钙素破片重组生产及其使用降钙素和有关的类似物生产。

    公开(公告)号：EP0892813A4

    公开(公告)日：2003-02-05

    申请号：EP97905717

    申请日：1997-02-04

    申请人： BIONEBRASKA INC

    发明人： WAGNER FRED W ,  STOUT JAY S ,  HENRIKSEN DENNIS B ,  PARTRIDGE BRUCE E ,  HOLMQUIST BART ,  FRANK JULIE A

    IPC分类号： C07K14/585 ,  C12N9/88 ,  C12N15/16 ,  A61K38/23

    CPC分类号： C07K14/585 ,  C07K2319/00 ,  C12N9/88

    摘要： A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.

    摘要翻译： 提供了一种用于降钙素片段的重组制备和降钙素和相关类似物的制备中的用途该片段的过程。 该方法包括形成重组的融合蛋白包括其中连接到碳酸酐降钙素片段。 所述重组FORMED融合蛋白随后裂解，产生的多肽包括降钙素片段。 因此，提供一种用于生产降钙素类似物卡巴包括聚光用重组FORMED降钙素片段的desaminononapeptide方法。

    TREATMENT OF ACUTE CORONARY SYNDROME WITH GLP-1
    5.
    发明公开
    TREATMENT OF ACUTE CORONARY SYNDROME WITH GLP-1 有权
    急性冠脉综合征用GLP-1治疗

    公开(公告)号：EP1282436A2

    公开(公告)日：2003-02-12

    申请号：EP01937500.5

    申请日：2001-05-18

    申请人： BIONEBRASKA, INC.

    发明人： COOLIDGE, Thomas, R. ,  EHLERS, Mario

    IPC分类号： A61K38/26 ,  A61P9/10

    CPC分类号： A61K38/26

    摘要： The invention relates to methods for treating a patient suffering from acute coronary syndrome, but who is not suffering from a Q-wave myocardial infarction, comprising administration of a therapeutically effective amount of a GLP-1 molecule. The GLP-1 can be self-administered, and can be administered in one or more doses, as needed, on an intermittent or continuous basis, to optimize metabolism in cardiac tissue and to prevent cardiac damage associated with ischemia.

    ENZYMATIC AMIDATION OF PEPTIDES
    8.
    发明公开
    ENZYMATIC AMIDATION OF PEPTIDES 审中-公开
    肽酶法酰胺化

    公开(公告)号：EP1127155A1

    公开(公告)日：2001-08-29

    申请号：EP99956920.5

    申请日：1999-11-05

    申请人： BIONEBRASKA, INC.

    发明人： DORMADY, Dan ,  STOUT, Jay, S. ,  STRYDOM, Daniel, J. ,  HOLMQUIST, Barton ,  WAGNER, Fred, W.

    IPC分类号： C12P21/06

    CPC分类号： C07K14/605 ,  C12P21/06

    摘要： The invention provides a method of producing a polypeptide having a C-terminal α-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an α-carboxamide group (C-terminal 'Arg-NH2'). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.

    METHOD FOR MODIFICATION OF RECOMBINANT POLYPEPTIDES
    9.
    发明公开
    METHOD FOR MODIFICATION OF RECOMBINANT POLYPEPTIDES 失效
    方法进行修改重组多肽。

    公开(公告)号：EP0651761A1

    公开(公告)日：1995-05-10

    申请号：EP93918187.0

    申请日：1993-07-13

    申请人： BIONEBRASKA, INC.

    发明人： STOUT, Jay ,  WAGNER, Fred W. ,  COOLIDGE, Thomas, R. ,  HOLMQUIST, Bart

    IPC分类号： C12N15 ,  C07K1 ,  C07K5 ,  C07K7 ,  C07K14 ,  C07K19 ,  C12P21

    CPC分类号： C07K14/60 ,  C07K1/003 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/1075 ,  C07K1/12 ,  C07K5/1019 ,  C07K7/18 ,  C07K14/4716 ,  C07K14/475 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/75 ,  C12N15/62 ,  Y02P20/55

    摘要： The invention provides for a chemical method for preparing a recombinant single copy polypeptide or a portion thereof with a modified terminal amino acid α-carbon reactive group selected from the group consisting of N-terminal α-amine, C-terminal α-carboxyl, and a combination thereof. The steps of the method involve forming the recombinant single copy polypeptide or a portion thereof so that the single copy polypeptide is protected with one or more biologically added protecting groups at the N-terminal α-amine, C-terminal α-carboxyl. The recombinant single copy polypeptide can then be reacted with up to three chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The recombinant single copy polypeptide can be cleaved with at least one cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid α-carbon reactive group. The unprotected terminal amino acid α-carbon reactive group is modified with at least one chemical modifying agent. The side chain protected terminally modified single copy polypeptide is then deprotected at the side chain groups to form a terminally modified recombinant single copy polypeptide. The number and sequence of steps in the method can be varied to achieve selective modification at the N- and/or C-terminal amino acid of a recombinantly produced polypeptide.