公开(公告)号：EP0217822A4

公开(公告)日：1989-05-11

申请号：EP86901613

申请日：1986-02-11

申请人： BIOTECH RES PARTNERS LTD

发明人： KUSHNER PETER J ,  COFER CLAIRE L ,  FRIEDMAN JEFFREY S ,  TALMADGE KAREN D

IPC分类号： C07K14/505 ,  C07K14/58 ,  C07K14/61 ,  C07K14/775 ,  C07K14/785 ,  C12N5/00 ,  C12N9/64 ,  C12N15/85

CPC分类号： C12N9/6486 ,  C07K14/505 ,  C07K14/58 ,  C07K14/61 ,  C07K14/775 ,  C07K14/785 ,  C12N15/85 ,  C12Y304/23015

摘要： An expression system for recombinant production of a desired protein comprises CHO cells transformed with a DNA sequence having the desired protein coding sequence under control of the human metallothionein-II promoter. The cells can be maintained on serum-free medium and induced in the presence of an induction mediator. In addition, the system may include an enhancer element and/or a resistance-conferring gene to provide increased levels of expression. The system can process genomic as well as intronless DNA, and is capable of producing proteins which have the same characteristics as those obtained from native sources. Human growth hormone which is indistinguishable from that produced by the pituitary has been thus produced. Expression systems containing genes encoding alveolar surfactant protein (ASH), apolipoproteins AI and AII (apoAI and apoAII), atrial natriuretic factor (ANF), erythropoetin (Epo), and renin are also contemplated.