公开(公告)号：EP0774009A1

公开(公告)日：1997-05-21

申请号：EP95926867.0

申请日：1995-07-13

申请人： BOEHRINGER INGELHEIM INTERNATIONAL GmbH

发明人： VON RÜDEN, Thomas ,  COTTEN, Matthew ,  WAGNER, Ernst ,  ZATLOUKAL, Kurt ,  LEHRMANN, Heike ,  PANZENBÖCK, Birgit

IPC分类号： C12N15 ,  A61K47 ,  C07K14 ,  C12N7 ,  C12N9

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N9/503 ,  C12N2710/10343 ,  C12N2740/13043

摘要： The invention concerns a process for preparing viruses and viral vectors by the transfection of mammal cells with complexes of viral DNA and a polycation, which is optionally conjugated with a ligand for the target cell, in the presence of an endosomolytic agent. The process affords particular advantages for the production of recombinant retroviruses, adenoviruses and adeno-associated viruses in the cell, permitting high virus titres in packing cell lines and in primary cells. The area of application is above all gene therapy.