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1.发明授权
公开(公告)号：EP0694071B1
公开(公告)日：2002-03-06
申请号：EP94913539.6
申请日：1994-04-06
发明人： COTTEN, Matthew , WAGNER, Ernst
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N15/87 , C12N2710/10323 , C12N2710/10343 , C12N2710/10345 , C12N2710/10362 , C12N2810/80
摘要： Described is an adenovirus whose surface has been modified with transferrin for the introduction of foreign DNA into higher eucaryotic cells, bonding preferably being through the glycoprotein part of the transferrin. The foreign DNA is imported into cells which have no adenovirus receptors and expressed. The adenovirus may be recombinant or non-recombinant.
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公开(公告)号：EP0724643A1
公开(公告)日：1996-08-07
申请号：EP94928873.0
申请日：1994-10-07
CPC分类号： C12N15/87 , A61K47/6901
摘要： An endosomolytically active virus-like particle composed of units of capside proteins of viruses or virus-like particles, in which the capside protein units have a membrane-active peptidic sequence. These particles are suitable as a component of preparations for the transport of nucleic acids into higher eucaryotic cells via receptor-mediated endocytoses.
摘要翻译： 由病毒的帽面蛋白单元或病毒样颗粒构成的内切解活性病毒样颗粒，其中帽侧蛋白单元具有膜活性肽序列。 这些颗粒适合作为通过受体介导的内吞将核酸运输到高等真核细胞中的制剂组分。
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3.发明公开
公开(公告)号：EP1224311A1
公开(公告)日：2002-07-24
申请号：EP00972745.4
申请日：2000-10-12
IPC分类号： C12N15/861 , A61K35/76
CPC分类号： C12N15/86 , C07K14/005 , C07K14/465 , C07K2319/20 , C12N7/00 , C12N2710/10221 , C12N2710/10222 , C12N2710/10243
摘要： Recombinant CELO virus or CELO virus DNA with a deletion or disruption of the Gam1 gene, optionally combined with deletions at the right or left end of the viral genome that allow insertion of large pieces of foreign DNA. The virus is useful as a vaccine for animals, in particular birds, and for gene therapy and vaccine applications in humans. The virus can also be used for recombinant protein production.
摘要翻译： 具有Gam1基因缺失或破坏的重组CELO病毒或CELO病毒DNA，任选地在病毒基因组的右端或左端结合缺失，允许插入大片外源DNA。 该病毒可用作动物特别是鸟类的疫苗，以及用于人类的基因治疗和疫苗应用。 该病毒也可用于重组蛋白质的生产。
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公开(公告)号：EP0689604A1
公开(公告)日：1996-01-03
申请号：EP94911921.0
申请日：1994-03-18
发明人： BIRNSTIEL, Max L. , BUSCHLE, Michael , COTTEN, Matthew , MAAS, Gerhard , PLANK, Christian , SCHAFFNER, Gotthold , SCHMIDT, Walter , WAGNER, Ernst , ZATLOUKAL, Kurt
IPC分类号： C12N15 , A61K35 , A61K39 , A61K47 , A61K48 , A61P35 , A61P37 , C07K14 , C12N5 , C12N7 , C12P21 , C12Q1 , A61K38 , C12R1
CPC分类号： C12N7/00 , A61K38/00 , A61K39/0011 , A61K47/50 , A61K48/00 , C07K14/005 , C07K14/485 , C07K14/535 , C07K14/55 , C07K14/57 , C07K14/705 , C12N15/87 , C12N2710/10343 , C12N2710/10361 , C12N2760/20122 , C12Q1/701
摘要： Cancer vaccines are prepared by treating tumor cells or fibroblasts with a complex of immunostimulating polypeptide-coding DNA and of DNA-binding substance, for example polylysine, preferably conjugated with transferrine. The complex further contains a conjugate of DNA-binding substance and of an endosomolytic peptide or an adenovirus having at least one E4 defect or one E1a defect associated with other genetic defects.
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5.发明公开NEUE, ÜBER ENDOZYTOSE IN HÖHERE EUKARYOTISCHE ZELLEN AUFNEHMBARE, NUKLEINSÄURE ENTHALTENDE KOMPLEXE 失效NEW，通过内吞作用进入更高的真核细胞账款，NUCLEIC包含复杂。
公开(公告)号：EP0571414A1
公开(公告)日：1993-12-01
申请号：EP92903641.0
申请日：1992-02-01
IPC分类号： A61K47
CPC分类号： A61K47/6883 , A61K47/645
摘要： Des complexes de conjugués de facteur de liaison et de facteur d'intériorisation d'une part, et d'acide nucléique d'autre part, susceptibles d'être assimilés par endocytose par des cellules eucaryotes supérieures, contiennent sous une forme liée par non-covalence une ou plusieurs substances ayant une affinité avec l'acide nucléique et capables d'augmenter l'absorbabilité des complexes par des cellules. Le cas échéant, la substance liée par non-covalence ayant une affinité avec l'acide nucléique est identique au facteur de liaison, de préférence une substance polycationique. Le facteur d'intériorisation est de préference la transferrine. L'invention concerne également un procédé de production de ces complexes et un procédé d'introduction d'acide nucléique dans des cellules eucaryotes supérieures, ainsi que des compositions pharmaceutiques contenant des complexes avec un acide nucléique à effet thérapeutique.
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公开(公告)号：EP0724643B1
公开(公告)日：2004-06-30
申请号：EP94928873.2
申请日：1994-10-07
CPC分类号： C12N15/87 , A61K47/6901
摘要： An endosomolytically active virus-like particle composed of units of capside proteins of viruses or virus-like particles, in which the capside protein units have a membrane-active peptidic sequence. These particles are suitable as a component of preparations for the transport of nucleic acids into higher eucaryotic cells via receptor-mediated endocytoses.
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公开(公告)号：EP0532525A1
公开(公告)日：1993-03-24
申请号：EP91909227.0
申请日：1991-05-10
IPC分类号： A61K31 , A61K39 , A61K47 , A61K48 , A61P31 , A61P35 , C07K14 , C07K16 , C07K17 , C07K19 , C12N5 , C12N15 , C12P21 , A61K38 , C12R1
CPC分类号： C12N15/87 , A61K38/00 , A61K47/645 , A61K47/6883 , A61K48/00 , A61K48/0033 , A61K48/0091 , C12N15/85
摘要： De nouveaux conjugués de protéines et de polycations sont capables de former avec des acides nucléiques et avec des analogues d'acides nucléiques des complexes solubles. La part protéique de ces conjugués est formée par une protéine capable de se lier à une protéine de la surface cellulaire exprimée par des cellules de la lignée des cellules T, de sorte que les complexes ainsi formés sont absorbés par des cellules qui expriment la protéine de surface des cellules T. Ces complexes utiles dans des compositions pharmaceutiques contiennent un acide nucléique thérapeutique ou actif en thérapie génétique.
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公开(公告)号：EP0774009A1
公开(公告)日：1997-05-21
申请号：EP95926867.0
申请日：1995-07-13
发明人： VON RÜDEN, Thomas , COTTEN, Matthew , WAGNER, Ernst , ZATLOUKAL, Kurt , LEHRMANN, Heike , PANZENBÖCK, Birgit
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N9/503 , C12N2710/10343 , C12N2740/13043
摘要： The invention concerns a process for preparing viruses and viral vectors by the transfection of mammal cells with complexes of viral DNA and a polycation, which is optionally conjugated with a ligand for the target cell, in the presence of an endosomolytic agent. The process affords particular advantages for the production of recombinant retroviruses, adenoviruses and adeno-associated viruses in the cell, permitting high virus titres in packing cell lines and in primary cells. The area of application is above all gene therapy.
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公开(公告)号：EP0750677A1
公开(公告)日：1997-01-02
申请号：EP95913883.0
申请日：1995-03-17
摘要： In a process for treating eucaryotic cells, in applications in which the toxicity of lipopolysaccharides has an adverse effect, substances are used during transfection or to clean the foreign material which bond lipopolysaccharides and neutralise their toxicity. The process is of particular advantage when foreign material is to be inserted in the cells, especially in gene transfer methods. Compositions containing a lipopolysaccharide-bonding substance are used in cell culture applications as a medium and in therapeutic applications as medicaments.
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10.发明公开
公开(公告)号：EP0694071A1
公开(公告)日：1996-01-31
申请号：EP94913539.0
申请日：1994-04-06
发明人： COTTEN, Matthew , WAGNER, Ernst
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N15/87 , C12N2710/10323 , C12N2710/10343 , C12N2710/10345 , C12N2710/10362 , C12N2810/80
摘要： Described is an adenovirus whose surface has been modified with transferrin for the introduction of foreign DNA into higher eucaryotic cells, bonding preferably being through the glycoprotein part of the transferrin. The foreign DNA is imported into cells which have no adenovirus receptors and expressed. The adenovirus may be recombinant or non-recombinant.
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