公开(公告)号：EP2591094A1

公开(公告)日：2013-05-15

申请号：EP11738351.3

申请日：2011-07-08

申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

发明人： GRILLBERGER, Leopold ,  REITER, Manfred ,  FLEISCHANDERL, Daniel ,  BRAMBERGER, Gregor

IPC分类号： C12N5/10 ,  C12N9/48 ,  C07K14/745 ,  A61K38/36

CPC分类号： C07K14/755 ,  A61K9/0019 ,  A61K38/00 ,  A61K38/36 ,  A61K38/37 ,  C12N9/6489 ,  C12N2500/24 ,  C12N2511/00 ,  C12P21/00 ,  C12Y304/24087

摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS 13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

摘要翻译： 在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS 13的细胞培养条件。 本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养上清液。 本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。