-
1.发明授权FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION 有权糖溶液进行不断的超速离心进行病毒清洁制剂
公开(公告)号：EP2155864B1
公开(公告)日：2013-07-17
申请号：EP08749278.1
申请日：2008-04-30
发明人： REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang , MITTERER, Artur , SCHAFHAUSER, Horst
CPC分类号： C12N7/00 , A61K39/00 , A61K39/12 , A61K39/145 , C12N2750/14351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252
摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
-
公开(公告)号：EP1520008B1
公开(公告)日：2012-09-05
申请号：EP03762657.9
申请日：2003-07-08
CPC分类号： C12N5/005 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , C12N5/0043 , C12N7/00 , C12N2500/74 , C12N2500/76 , C12N2710/24134 , C12N2760/16134 , C12N2770/36134 , Y02A50/388 , Y02A50/39 , Y02A50/394 , Y02A50/396 , Y02A50/466
摘要： The present invention relates to a methods for producing a virus or an immunogenic composition comprising a virus or virus antigen using a culture of cells that have been grown in an animal protein free medium comprising soy hydrolysate and yeast hydrolysate.
-
公开(公告)号：EP2459702A1
公开(公告)日：2012-06-06
申请号：EP10740810.6
申请日：2010-07-30
发明人： GRILLBERGER, Leopold , SPENGER, Alexandra , HASSLACHER, Meinhard , GRILLBERGER, Rana , REITER, Manfred
CPC分类号： C12N9/6489 , C12N5/0682 , C12N5/0686 , C12N2500/14 , C12N2500/22 , C12N2500/38 , C12N2500/95 , C12N2500/99 , C12N2510/02 , C12Y304/24087
摘要： The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.
-
4.发明公开METHOD OF PRODUCING RECOMBINANT ADAMTS13 IN CELL CULTURE 审中-公开VERFAHREN ZUR HERSTELLUNG VON REKOMBINANTEM ADAMTS13 IN EINER ZELLKULTUR
公开(公告)号：EP2591094A1
公开(公告)日：2013-05-15
申请号：EP11738351.3
申请日：2011-07-08
IPC分类号： C12N5/10 , C12N9/48 , C07K14/745 , A61K38/36
CPC分类号： C07K14/755 , A61K9/0019 , A61K38/00 , A61K38/36 , A61K38/37 , C12N9/6489 , C12N2500/24 , C12N2511/00 , C12P21/00 , C12Y304/24087
摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS 13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
摘要翻译： 在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS 13的细胞培养条件。 本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养上清液。 本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。
-
5.发明公开METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE 有权ZELLKULTUR VERFAHREN ZUR HERSTELLUNG VON HOCHMOLEKULAREM VON WILLEBRAND FAKTOR
公开(公告)号：EP2590998A1
公开(公告)日：2013-05-15
申请号：EP11736502.3
申请日：2011-07-08
IPC分类号： C07K14/755 , C12N5/00 , A61K38/36 , C12N5/10
CPC分类号： C07K14/755 , A61K9/0019 , A61K38/00 , A61K38/36 , A61K38/37 , C12N9/6489 , C12N2500/24 , C12N2511/00 , C12P21/00 , C12Y304/24087
摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH
4
+ ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.摘要翻译： 在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS 13的细胞培养条件。 本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养上清液。 本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。
-
6.发明授权INACTIVATION OF A PATHOGEN IN A SAMPLE BY A TREATMENT WITH FORMALIN AND UV LIGHT 有权致病菌的在样品通过用福尔马林和紫外线灭活
公开(公告)号：EP1753470B1
公开(公告)日：2015-08-19
申请号：EP05741342.9
申请日：2005-05-26
CPC分类号： A61L2/0088 , A61L2/0011
-
公开(公告)号：EP2155863B1
公开(公告)日：2014-08-13
申请号：EP08749279.9
申请日：2008-04-30
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5252 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251 , C12N2770/24151 , C12N2770/36151 , Y02A50/394
摘要： The present invention provides a method for the production of a virus. The method includes providing a host cell that has been infected by the virus and cultivating the infected host cell at two different temperatures. The virus produced by the cultivation steps is subsequently collected. By using the dual temperature cultivation process, high titer and improved purity can be obtained.
-
8.发明公开SUBSTANTIALLY ANIMAL PROTEIN-FREE RECOMBINANT FURIN AND METHODS FOR PRODUCING SAME 审中-公开WEITGEHEND TIERPROTEINFREIES REKOMBINANTES FURIN UND HERSTELLUNGSVERFAHRENDAFÜR
公开(公告)号：EP2240578A1
公开(公告)日：2010-10-20
申请号：EP08869658.8
申请日：2008-12-19
发明人： PLAIMAUER, Barbara , VON FIRCKS, Simone , GRILLBERGER, Leopold , HASSLACHER, Meinhard , GEYER, Roland , MITTERER, Artur , REITER, Manfred
IPC分类号： C12N9/64 , C07K14/755 , C12P21/02
CPC分类号： C12N9/6454
摘要： The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.
摘要翻译： 本发明涉及重组弗林蛋白酶（rFurin）和生产rFurin的方法。 更具体地，本发明涉及基本上无动物蛋白的rFurin和用于生产基本上无动物蛋白的rFurin的方法。
-
9.发明公开FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION 有权糖溶液进行不断的超速离心进行病毒清洁制剂
公开(公告)号：EP2155864A2
公开(公告)日：2010-02-24
申请号：EP08749278.1
申请日：2008-04-30
发明人： REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang , MITTERER, Artur , SCHAFHAUSER, Horst
CPC分类号： C12N7/00 , A61K39/00 , A61K39/12 , A61K39/145 , C12N2750/14351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252
摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
-
公开(公告)号：EP2155863A1
公开(公告)日：2010-02-24
申请号：EP08749279.9
申请日：2008-04-30
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5252 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251 , C12N2770/24151 , C12N2770/36151 , Y02A50/394
摘要： The present invention provides a method for the production of a virus. The method includes providing a host cell that has been infected by the virus and cultivating the infected host cell at two different temperatures. The virus produced by the cultivation steps is subsequently collected. By using the dual temperature cultivation process, high titer and improved purity can be obtained.
-
-
-
-
-
-
-
-
-
搜索结果
27 条记录 (耗时 0.017 秒)
