利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

4 条记录 (耗时 0.011 秒)

    Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof
    2.
    发明公开
    Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof 审中-公开
    包含高度亲水性的非核苷基团与多个标记以及它们的用途的核酸探针

    公开(公告)号：EP1136569A3

    公开(公告)日：2004-01-28

    申请号：EP01105433.5

    申请日：2001-03-13

    申请人： Bayer Corporation

    发明人： Yang,, Guohan ,  Ford, Donna M. ,  Law, Say-Jong ,  Monahan, John E. ,  Sells, Todd B.

    IPC分类号： C12Q1/68 ,  C07H21/00

    CPC分类号： C07H21/04 ,  C07H21/00

    摘要： Highly hydrophilic non-nucleosidic tags with multiple labels are provided for use in nucleic acid probes. The tags are branched structures having a phosphodiester backbone, which have the advantages of a small dimensional size and high hydrophilicity. After the tag is labeled, its high negative charge and minimal size help to keep the carriers away from DNA or RNA molecules, due to repulsion between negative charges. Non-specific intercalation and steric hindrance are therefore minimized, and the hydrophobicity, if any of reporter molecules is reduced. The probes are used in place of conventionally labeled oligonucleotides for a variety of hybridization reactions.

    Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof
    3.
    发明公开
    Nucleic acid probes having highly hydrophilic non-nucleosidic tags with multiple labels, and uses thereof 审中-公开
    包含高度亲水性的非核苷基团与多个标记以及它们的用途的核酸探针

    公开(公告)号：EP1136569A2

    公开(公告)日：2001-09-26

    申请号：EP01105433.5

    申请日：2001-03-13

    申请人： Bayer Corporation

    发明人： Yang,, Guohan ,  Ford, Donna M. ,  Law, Say-Jong ,  Monahan, John E. ,  Sells, Todd B.

    IPC分类号： C12Q1/68

    CPC分类号： C07H21/04 ,  C07H21/00

    摘要： Highly hydrophilic non-nucleosidic tags with multiple labels are provided for use in nucleic acid probes. The tags are branched structures having a phosphodiester backbone, which have the advantages of a small dimensional size and high hydrophilicity. After the tag is labeled, its high negative charge and minimal size help to keep the carriers away from DNA or RNA molecules, due to repulsion between negative charges. Non-specific intercalation and steric hindrance are therefore minimized, and the hydrophobicity, if any of reporter molecules is reduced. The probes are used in place of conventionally labeled oligonucleotides for a variety of hybridization reactions.

    摘要翻译： 提供了用于在核酸探针与使用多个标签高度亲水性的非核苷标签。 这种标签是具有磷酸二酯骨架的支链结构，其中有一个小的尺寸大小和亲水性高的优点。 标签标记后，其高负电荷和最小尺寸的帮助，从DNA或RNA分子保持运营商了，由于负电荷之间的排斥。 特定的非插层和空间位阻，因此最小化，并且疏水性，如果有的话报道分子的降低。 探针代替常规标记寡核苷酸用于各种杂交反应。