公开(公告)号：EP1682865B1

公开(公告)日：2018-08-15

申请号：EP04816916.3

申请日：2004-10-08

申请人： Beckman Coulter, Inc.

发明人： ORTIZ, Nery ,  LI, Jing ,  LI, Yi ,  SOCARRAS, Sandra

IPC分类号： G01N1/28 ,  G01N15/10 ,  G01N15/12 ,  G01N33/80 ,  G01N33/96

CPC分类号： G01N15/12 ,  G01N15/1012 ,  G01N33/80 ,  G01N33/96 ,  G01N2001/2893 ,  G01N2015/1037 ,  G01N2496/05

摘要： Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.

公开(公告)号：EP2082224B1

公开(公告)日：2015-05-13

申请号：EP07868731.6

申请日：2007-11-13

申请人： Beckman Coulter, Inc.

发明人： ORTIZ, Nery ,  GERULA, Theodore J.

IPC分类号： G01N33/49 ,  G01N33/96

CPC分类号： G01N33/96 ,  G01N15/12 ,  G01N2015/1006 ,  G01N2496/10 ,  Y10T436/10 ,  Y10T436/101666

公开(公告)号：EP1412739B1

公开(公告)日：2008-12-31

申请号：EP02748163.9

申请日：2002-07-15

申请人： Beckman Coulter, Inc.

发明人： ELLIOTT, Michael ,  JACOBS, Dana, B. ,  ORTIZ, Nery ,  GOLDSON, Wayne ,  WHITLEY, Penny ,  GERULA, Theodore, J.

IPC分类号： G01N31/00

CPC分类号： G01N33/96 ,  G01N2496/05 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831 ,  Y10T436/106664 ,  Y10T436/108331

摘要： An improved blood suspension media for hematological compositions, having particular utility with a red blood cell component for devices using electronic and optical means for blood determinations, and processes for using the suspension media. The suspension media finds particular utility in providing the hematology control product with a stable and consistent MCV and RDW for an extended product shelf life.

公开(公告)号：EP2002256B1

公开(公告)日：2017-10-18

申请号：EP07757394.7

申请日：2007-02-23

申请人： Beckman Coulter, Inc.

发明人： ORTIZ, Nery ,  GERULA, Theodore, J. ,  SOCARRAS, Sandra

IPC分类号： G01N31/00 ,  G01N33/96 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14

CPC分类号： G01N33/96 ,  G01N15/1012 ,  G01N15/12 ,  G01N15/147 ,  G01N2015/1037 ,  G01N2496/05 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/2525

公开(公告)号：EP1682865A2

公开(公告)日：2006-07-26

申请号：EP04816916.3

申请日：2004-10-08

申请人： Beckman Coulter, Inc.

发明人： ORTIZ, Nery ,  LI, Jing ,  LI, Yi ,  SOCARRAS, Sandra

IPC分类号： G01N1/00

CPC分类号： G01N15/12 ,  G01N15/1012 ,  G01N33/80 ,  G01N33/96 ,  G01N2001/2893 ,  G01N2015/1037 ,  G01N2496/05

摘要： Methods of using a reference control composition containing a nucleated red blood cell component for measurement of nucleated red blood cells are disclosed. The nucleated red blood cell component is made of stabilized or processed nucleated blood cells which have impedance and optical properties simulating the impedance and optical properties of human nucleated red blood cells under a blood lysing condition as measured by a specific measurement. The methods include mixing the reference control composition with a lytic reagent, and analyzing the control sample mixture on a flow cytometric instrument by impedance, impedance and optical measurement, or DC impedance and a multi-dimensional measurement of light scatter, radio frequency and a second DC impedance; and reporting the simulated nucleated red blood cells in the reference control composition.

公开(公告)号：EP1668353A1

公开(公告)日：2006-06-14

申请号：EP04785312.2

申请日：2004-09-30

申请人： Beckman Coulter, Inc.

发明人： LI, Yi ,  ORTIZ, Nery ,  YOUNG, Carole, J. ,  GALVEZ, Santiago

IPC分类号： G01N31/00 ,  G01N21/00

CPC分类号： G01N15/1459 ,  G01N33/56972 ,  G01N33/80 ,  G01N33/96 ,  G01N2015/0073 ,  G01N2015/1062 ,  G01N2015/1486 ,  G01N2015/1493 ,  G01N2496/10 ,  Y10T436/10 ,  Y10T436/101666

摘要： Reference control compositions and the method of use are disclosed for measurement of nucleated red blood cells, which includes one set of synthetic spherical particles having a mean particle diameter ranging from 6.2 µm to 6.8 µm and a refractive index from 1.58 to 1.62 monodispersed in an aqueous suspension medium. The synthetic spherical particles have optical properties simulating optical properties of nucleated red blood cells as measured by optical measurements. The reference control composition can further include a second set of the synthetic spherical particles having optical properties simulating optical properties of white blood cells. Further disclosed is a reference control system which includes a series of reference control compositions, each having an amount of one type of synthetic spherical particles which have optical properties simulating the optical properties of nucleated red blood cells having a specific cell maturity.

公开(公告)号：EP3794346A1

公开(公告)日：2021-03-24

申请号：EP19729962.1

申请日：2019-05-17

申请人： Beckman Coulter, Inc.

发明人： FIGUEROA, Ignacio ,  LI, Jing ,  ORTIZ, Nery ,  GOLDSON, Wayne

IPC分类号： G01N33/50 ,  G01N33/72 ,  G01N33/80

公开(公告)号：EP3701541A1

公开(公告)日：2020-09-02

申请号：EP18845383.1

申请日：2018-10-26

申请人： Beckman Coulter Inc.

发明人： RAMIREZ, Carlos ,  ORTIZ, Nery ,  SWABY, Milton

IPC分类号： G16H40/40 ,  G16H10/40 ,  G16H40/60 ,  G16H50/20

公开(公告)号：EP1766045B1

公开(公告)日：2010-12-01

申请号：EP05745792.1

申请日：2005-05-04

申请人： Beckman Coulter, Inc.

发明人： ORTIZ, Nery ,  GERULA, Theodore, J. ,  LI, Yi

IPC分类号： G01N31/00 ,  G01N33/53

CPC分类号： G01N33/96 ,  G01N15/12 ,  G01N2015/1006 ,  Y10S435/967 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664

摘要： A hematology reference control containing an immature granulocyte component and the method of making are disclosed. The reference control includes an immature granulocyte component made of processed blood cells for simulating human immature granulocytes, and a suspension medium suitable for delivering the component to a blood analyzer for measurement of immature granulocytes. The immature granulocyte component can be made of processed avian, reptile or fish red blood cells, or human immature granulocytes produced in vitro. The reference control further includes a white blood cell component which includes white blood cell sub-components for simulating white blood subpopulations. Further disclosed is the method of using the reference control for measurement of immature granulocytes on a blood analyzer.

公开(公告)号：EP1738163A1

公开(公告)日：2007-01-03

申请号：EP05735364.1

申请日：2005-03-30

申请人： Beckman Coulter, Inc.

发明人： ORTIZ, Nery ,  SOCARRAS, Sandra

IPC分类号： G01N31/00 ,  G01N33/48

CPC分类号： G01N33/96 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2496/05 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/106664 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/25 ,  Y10T436/25125

摘要： A method of making a reference control containing a nucleated red blood cell component includes providing a blood cell containing a nucleus; treating the blood cell with a treatment solution to alter a nucleus property from a natural value to a target value suitable for simulating nucleated red blood cells on a blood analyzer; and suspending treated blood cell in a suspension medium to form a reference control. The method also includes integrating the nucleated red blood cell component with white blood cell, red blood cell, platelet and reticulocyte components. Further disclosed is a cell treatment composition for altering a nucleus property, which includes a conditioning component, a lytic component for permeating cell membrane, and a fixing component for preserving the cell nucleus. Also disclosed is a method of using the reference control for measurement of nucleated red blood cells on a blood analyzer.