公开(公告)号：EP2834367A1

公开(公告)日：2015-02-11

申请号：EP13773119.6

申请日：2013-04-04

申请人： Becton Dickinson and Company

发明人： LI, Xiao ,  ZHANG, Yongqiang ,  KOPHER, Kenneth, Anthony ,  MANTLO, John, D. ,  POPE, William, Alfred ,  SHI, Song ,  YUP, Axel, A.

IPC分类号： C12Q1/02 ,  C12Q1/68 ,  G01N33/48 ,  C12M1/34

CPC分类号： C12Q1/06 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2563/173 ,  C12Q2565/626

摘要： Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

公开(公告)号：EP2971050A1

公开(公告)日：2016-01-20

申请号：EP13878493.9

申请日：2013-04-04

申请人： Becton, Dickinson and Company

发明人： LI, Xiao ,  KOPHER, Kenneth, Anthony ,  MANTLO, John, D. ,  POPE, William, Alfred ,  SHI, Song ,  YUP, Axel, A.

IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/48

CPC分类号： C12Q1/6806 ,  C12Q1/06 ,  C12Q2527/125 ,  C12Q2563/173 ,  C12Q2565/626

摘要： A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

摘要翻译： 确定样品中微生物（例如细菌）存在，不存在或总量之一的方法。 根据该过程，获得含有复杂基质的生物样品。 首先将样品与树脂结合以从样品中吸附复合基质。 从生物样品中除去树脂。 然后通过流式细胞术分析如此制备的样品。