公开(公告)号：EP2393519B1

公开(公告)日：2018-01-03

申请号：EP10704093.3

申请日：2010-02-04

申请人： Bicycle Therapeutics Limited

发明人： WINTER, Gregory Paul ,  HEINIS, Christian ,  BERNARD, Elise ,  LOAKES, David ,  TITE, John Paul ,  VAYSBURD, Marina ,  TEUFEL, Daniel Paul ,  RIECHMANN, Lutz

IPC分类号： G01N33/68 ,  C07K14/00 ,  C12N15/10

CPC分类号： C12N15/1037 ,  A61K47/55 ,  A61K47/64 ,  A61K47/643 ,  C07K14/001 ,  C12N15/1044 ,  C12N15/1058 ,  C40B40/08 ,  C40B40/10 ,  C40B50/06 ,  G01N33/531

摘要： The invention relates to a method for modifying one or more peptide ligands, comprising polypeptides covalently linked to a molecular scaffold at two or more amino acid residues, comprising the steps of providing one or more peptide ligands, wherein the polypeptide comprises two or more reactive groups which form a covalent linkage to the molecular scaffold, and at least one loop which comprises a sequence of two or more amino acids subtended between two of said reactive groups; exposing the peptide ligands to one or more proteases; and sorting the ligands according to the extent of proteolytic cleavage.