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公开(公告)号：EP1146961A1
公开(公告)日：2001-10-24
申请号：EP00967055.5
申请日：2000-09-29
申请人： Biomerieux, Inc.
发明人： KLUTTZ, Brian , BURG, Lawrence , TEGELER, Garry , GRAZIANO, Louis , COTTER, Christopher , GUY, Michel , GREER, James , MCKINLEY, Geoff, A. , CATANZARITI, Luigi , GLASSFIELD, Robert , BISHOP, James, Clement , COLIN, Bruno , PARIS, Cecile , WANG, Thomas , MORIN, Michael
IPC分类号： B01L3/00
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/026 , B01L2300/0825 , B01L2300/0861 , B01L2400/049 , B01L2400/0644 , B01L2400/0655 , G01N2035/00366
摘要： A unit dose test device for a nucleic acid amplification reaction has the form of an elongate disposable test strip. The test strip includes a dual-chamber reaction vessel preloaded with nucleic acid amplication reaction agents, a plurality of wells for processing a reaction occuring in the reaction vessel, and a hinge cover above a membrane, sealing both reaction chambers. A desiccant is loaded in proximity to and in communication with first or second reaction chamber.
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公开(公告)号：EP1146963B1
公开(公告)日：2005-03-02
申请号：EP00968466.3
申请日：2000-09-29
申请人： Biomerieux, Inc.
发明人： KLUTTZ, Brian , McKINLEY, Geoff, A. , GENNARI, Fabio , GUY, Michel , COTTER, Christopher , CATANZARITI, Luigi , GRAZIANO, Louis , COLIN, Bruno , PARIS, Cécile , DACHAUD, Jacques
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
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公开(公告)号：EP1146961B1
公开(公告)日：2007-09-05
申请号：EP00967055.5
申请日：2000-09-29
申请人： Biomerieux, Inc.
发明人： KLUTTZ, Brian , BURG, Lawrence , TEGELER, Garry , GRAZIANO, Louis , COTTER, Christopher , GUY, Michel , GREER, James , MCKINLEY, Geoff, A. , CATANZARITI, Luigi , GLASSFORD, Robert , BISHOP, James, Clement , COLIN, Bruno , PARIS, Cecile , WANG, Thomas , MORIN, Michael
IPC分类号： B01L3/00
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/026 , B01L2300/0825 , B01L2300/0861 , B01L2400/049 , B01L2400/0644 , B01L2400/0655 , G01N2035/00366
摘要： A unit dose test device for a nucleic acid amplification reaction has the form of an elongate disposable test strip. The test strip includes a dual-chamber reaction vessel preloaded with nucleic acid amplication reaction agents, a plurality of wells for processing a reaction occuring in the reaction vessel, and a hinge cover above a membrane, sealing both reaction chambers. A desiccant is loaded in proximity to and in communication with first or second reaction chamber.
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公开(公告)号：EP1146963A1
公开(公告)日：2001-10-24
申请号：EP00968466.3
申请日：2000-09-29
申请人： Biomerieux, Inc.
发明人： KLUTTZ, Brian , McKINLEY, Geoff, A. , GENNARI, Fabio , GUY, Michel , COTTER, Christopher , CATANZARITI, Luigi , GRAZIANO, Louis , COLIN, Bruno , PARIS, Cécile , DACHAUD, Jacques
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
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