公开(公告)号：EP4050096A1

公开(公告)日：2022-08-31

申请号：EP19950145.3

申请日：2019-12-17

申请人： Bloomage Biotechnology Corporation Limited ,  Jiangnan University

发明人： KANG, Zhen ,  CHEN, Jian ,  HU, Litao ,  DU, Guocheng ,  WANG, Yang ,  LI, Jianghua ,  LI, Jialian ,  ZHANG, Tianmeng

IPC分类号： C12N1/21 ,  C12N15/77 ,  C12P19/26 ,  C12R1/15

摘要： Disclosed is a high-efficiency synthesis and high-purity hyaluronic acid, and a recombinant corynebacterium glutamicum for an oligosaccharide thereof, relating to the technical field of bio-engineering. The yield of hyaluronic acid produced by the constructed recombinant corynebacterium glutamicum reaches 40 g/L, and the purity of the crude product reaches 95%. By means of a hyaluronic acid hydrolase from an external source and optimizing fermentation conditions, hyaluronic acid oligosaccharide of a specific molecular weight can be obtained, and further increasing of hyaluronic acid yield can be achieved, causing the yield to reach 72 g/L.