公开(公告)号：EP0983511A4

公开(公告)日：2001-12-05

申请号：EP97908968

申请日：1997-03-03

申请人： CALIFORNIA INST OF TECHN

发明人： ARNOLD FRANCES H ,  GUAN ZHIBIN ,  CHEN CHAO-TSEN ,  CHEN GUOHUA

IPC分类号： C12Q1/00 ,  G01N33/66 ,  G01N33/68

CPC分类号： G01N33/68 ,  C12Q1/006 ,  G01N33/66 ,  G01N2600/00 ,  G01N2650/00

摘要： Sensors (20, 50, 70) for use in detecting the presence of sugars and other analytes (target molecules). The sensor is composed of a metal complex that binds to the target molecule and releases a proton or includes an exchangable ligand which is exchanged for the target molecule during the binding interaction between the metal complex and the target molecule. The result of the binding interaction is the release of a proton, hydroxide ion or ligand species generated during the ligand exchange. Measurement of the release of proton, hydroxide ion or other ligand species from the sensor (20, 50, 70) provides an indirect indication of target molecule concentration. The metal complexes may be attached to support structures (10, 12) to provide both anchoring and positioning of the metal ions to increase selectivity of sugar/metal complex interactions. Detection systems in which pH is used as an indication of proton or hydroxide release are disclosed, as are detection systems in which Cl release is used. Methods for monitoring the concentrations of sugars and related molecules using the metal based sensors (20, 50, 70) are also disclosed.