公开(公告)号：EP1036198B1

公开(公告)日：2012-09-26

申请号：EP98960709.8

申请日：1998-12-04

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： ARNOLD, Frances ,  SHAO, Zhixin ,  VOLKOV, Alexander

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N15/10

CPC分类号： C12N15/1058 ,  C07K14/43595 ,  C12N15/1027 ,  C12N15/1034

公开(公告)号：EP1036198A1

公开(公告)日：2000-09-20

申请号：EP98960709.8

申请日：1998-12-04

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： ARNOLD, Frances ,  SHAO, Zhixin ,  VOLKOV, Alexander

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N15/1058 ,  C07K14/43595 ,  C12N15/1027 ,  C12N15/1034

摘要： The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.