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1.发明公开Expression of g-csf and muteins thereof and their use 失效表达von G-CSF，Muteine davon und deren Verwendung。
公开(公告)号：EP0256843A1
公开(公告)日：1988-02-24
申请号：EP87307114.6
申请日：1987-08-11
申请人： CETUS CORPORATION
CPC分类号： C07K14/535 , A61K38/00
摘要： Human recombinant G-CSF (hr-G-CSF), an mRNA fraction encoding hr-G-CSF, a DNA sequence encoding hr-G-CSF, modified DNA sequences encoding hr-G-CSF, that are expressed at high levels in recombinant hosts are disclosed. Modifications to the 5' DNA sequence encoding mature mG-CSF are also disclosed. The modifications to the third position nucleotide of at least one in the 5' region codon of the DNA sequence of G-CSF result in enhanced expression of mG-CSF protein in recombinant host cells, without changing the amino acid sequence of the protein. Other modifications yield muteins of G-CSF in which the NH 2 -terminal amino acid is not methionine when produced from a recombinant host cell. Expression vectors carrying the altered mG-CSF DNA sequence are disclosed and claimed.
摘要翻译： 人重组G-CSF（hr-G-CSF），编码hr-G-CSF的mRNA级分，编码hr-G-CSF的DNA序列，编码hr-G-CSF的修饰DNA序列，以高水平表达 公开了重组宿主。 还公开了编码成熟mG-CSF的5分钟DNA序列的修饰。 在G-CSF的DNA序列的5分钟区域密码子中至少一个的第三位核苷酸的修饰导致重组宿主细胞中mG-CSF蛋白的表达增强，而不改变蛋白质的氨基酸序列。 当由重组宿主细胞产生时，其它修饰产生G-CSF的突变蛋白，其中NH2末端氨基酸不是甲硫氨酸。 携带改变的mG-CSF DNA序列的表达载体被公开并要求保护。
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2.发明公开Plasminogen activators, DNA encoding the same and their preparation and use 失效PLASMINOGEN激活剂，编码它们的DNA及其制备和使用
公开(公告)号：EP0273774A3
公开(公告)日：1988-12-14
申请号：EP87311532
申请日：1987-12-30
申请人： CETUS CORPORATION
CPC分类号： C12N9/6456
摘要： Novel plasminogen activators are disclosed. Of the novel plasminogen activators, one is a modified urokinase that is altered to make it less susceptible to forming two chain-urokinase and to increase the fibrin specificity of urokinase. Also among the novel plasminogen activators are fibrinolytic proteins comprised of at least one domain capable of interacting with fibrin and the protease domain of urokinase. The domain capable of interacting with fibrin may have an amino acid sequences substantially the same as those of kringle-2 of t-PA, kringle-1 and/or -4 of plasminogen and the finger domain of t-PA. The protease domain may have an amino acid sequence substantially the same as that of 30 Kd urokinase. DNA sequences encoding the novel plasminogen activators, expression vectors and expression systems therefor are also disclosed and claimed.
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3.发明公开Plasminogen activators, DNA encoding the same and their preparation and use 失效Plasminogenaktivatoren，dafürkodierende DNS，ihre Herstellung und Anwendung。
公开(公告)号：EP0273774A2
公开(公告)日：1988-07-06
申请号：EP87311532.3
申请日：1987-12-30
申请人： CETUS CORPORATION
CPC分类号： C12N9/6456
摘要： Novel plasminogen activators are disclosed. Of the novel plasminogen activators, one is a modified urokinase that is altered to make it less susceptible to forming two chain-urokinase and to increase the fibrin specificity of urokinase. Also among the novel plasminogen activators are fibrinolytic proteins comprised of at least one domain capable of interacting with fibrin and the protease domain of urokinase. The domain capable of interacting with fibrin may have an amino acid sequences substantially the same as those of kringle-2 of t-PA, kringle-1 and/or -4 of plasminogen and the finger domain of t-PA. The protease domain may have an amino acid sequence substantially the same as that of 30 Kd urokinase. DNA sequences encoding the novel plasminogen activators, expression vectors and expression systems therefor are also disclosed and claimed.
摘要翻译： 公开了新型纤溶酶原激活剂。 在新型纤溶酶原激活剂中，一种是改变的尿激酶，其被改变以使其不易形成两种尿链激酶，并增加尿激酶的纤维蛋白特异性。 新型纤溶酶原激活剂之一也是由至少一个能够与纤维蛋白相互作用的结构域和尿激酶蛋白酶结构域组成的纤维蛋白溶解性蛋白质。 能够与纤维蛋白相互作用的结构域可以具有与纤溶酶原的t-PA，kringle-1和/或-4以及t-PA的手指结构域的kringle-2基本相同的氨基酸序列。 蛋白酶结构域可以具有与30Kd尿激酶基本相同的氨基酸序列。 还公开并要求保护编码新型纤溶酶原激活剂的DNA序列，表达载体及其表达系统。
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