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公开(公告)号：EP0272779A3
公开(公告)日：1988-08-03
申请号：EP87309409
申请日：1987-10-23
申请人： CETUS CORPORATION
发明人： Koths, Kirston Edward , Halenbeck, Robert Forgan , Kawasaki, Ernest Seigo , Ladner, Martha Ballie , Coyne, Mazie Yee , van Arsdell, Janelle Nobel , Martin, George Arthur
摘要： A colony stimulating factor, CSF-1, is a lymphokine useful in regulating the immune system. CSF-1 is obtained in usable amounts by recombinant methods, including cloning and expression of the murine and human DNA sequences encoding this protein. Both "long" and "short" forms of this protein and muteins corresponding to the cDNA-encoded forms are disclosed.
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2.发明公开Expression of g-csf and muteins thereof and their use 失效表达von G-CSF，Muteine davon und deren Verwendung。
公开(公告)号：EP0256843A1
公开(公告)日：1988-02-24
申请号：EP87307114.6
申请日：1987-08-11
申请人： CETUS CORPORATION
CPC分类号： C07K14/535 , A61K38/00
摘要： Human recombinant G-CSF (hr-G-CSF), an mRNA fraction encoding hr-G-CSF, a DNA sequence encoding hr-G-CSF, modified DNA sequences encoding hr-G-CSF, that are expressed at high levels in recombinant hosts are disclosed. Modifications to the 5' DNA sequence encoding mature mG-CSF are also disclosed. The modifications to the third position nucleotide of at least one in the 5' region codon of the DNA sequence of G-CSF result in enhanced expression of mG-CSF protein in recombinant host cells, without changing the amino acid sequence of the protein. Other modifications yield muteins of G-CSF in which the NH 2 -terminal amino acid is not methionine when produced from a recombinant host cell. Expression vectors carrying the altered mG-CSF DNA sequence are disclosed and claimed.
摘要翻译： 人重组G-CSF（hr-G-CSF），编码hr-G-CSF的mRNA级分，编码hr-G-CSF的DNA序列，编码hr-G-CSF的修饰DNA序列，以高水平表达 公开了重组宿主。 还公开了编码成熟mG-CSF的5分钟DNA序列的修饰。 在G-CSF的DNA序列的5分钟区域密码子中至少一个的第三位核苷酸的修饰导致重组宿主细胞中mG-CSF蛋白的表达增强，而不改变蛋白质的氨基酸序列。 当由重组宿主细胞产生时，其它修饰产生G-CSF的突变蛋白，其中NH2末端氨基酸不是甲硫氨酸。 携带改变的mG-CSF DNA序列的表达载体被公开并要求保护。
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