公开(公告)号：EP1831345A2

公开(公告)日：2007-09-12

申请号：EP05853864.6

申请日：2005-12-13

申请人： CHOLESTECH CORPORATION

发明人： BELLET, Neal, F. ,  SHINDELMAN, Jeffrey ,  WORTHY, Thomas, E. ,  HALEY, Kimberly ,  LEE, Richard, K-T ,  KYUNG, Jane, Eunhee ,  QIAN, Suyue

IPC分类号： C12M1/34

CPC分类号： G01N21/03 ,  B01L3/5023 ,  B01L9/52 ,  B01L2200/0668 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0633 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/54386 ,  G01N2035/00108 ,  G01N2035/00128 ,  G01N2035/0436 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S435/97 ,  Y10S436/81

摘要： An immunoassay cassette, apparatus, and method for detecting an analyte in a liquid body-fluid sample are disclosed. The cassette has a body and a support mounted on the body, for movement relative to the body to first and second transfer positions. Sample supplied to a sample well in the cassette body is transferred to a reagent reservoir on the cassette body, by moving the support to its first transfer position. Here the sample reacts with a first reagent composition in the reservoir effective to form a modified sample. The modified sample is then transferred to a reagent strip on the support by moving the support to its second transfer position. The reagent strip has a transfer zone at which sample material is transferred to the strip, a second reagent composition effective to react with the modified sample to form a detectable analyte­-dependent product, and a detection zone located downstream at which the detectable product can be observed. By controlling movement of the support to its first and second positions, the cassette can effectively handle a variety of samples having different flow rates.