公开(公告)号：EP0519866A1

公开(公告)日：1992-12-23

申请号：EP92810445.4

申请日：1992-06-10

申请人： CIBA-GEIGY AG

发明人： Mestan, Jürgen, Dr. ,  Lazdins, Janis K., Dr. ,  Woods-Cook, Kathie A. ,  Hardman, Norman, Dr. ,  Hochkeppel, Heinz-Kurt, Dr.

IPC分类号： C12N15/13 ,  C12P21/08 ,  C12N15/63 ,  C12N5/10 ,  C12N5/20 ,  A61K39/42 ,  G01N33/569 ,  G01N33/577

CPC分类号： C07K16/1054 ,  A61K38/00 ,  C07K16/462 ,  C07K2317/24 ,  C07K2317/732

摘要： The invention concerns monoclonal antibodies and antibody derivatives directed against HIV core protein p24 which recognize p24 expressed on the surface of HIV-infected macrophages and/or kill HIV-infected cells. The monoclonal antibodies of the invention may be murine antibodies or chimeric antibodies consisting of human constant regions and murine variable or hypervariable regions. Methods of manufacture of such antibodies, hybridoma or transfectoma cell lines secreting them and methods for production of the hybridoma or transfectoma cell lines are also encompassed by this invention. The invention further concerns recombinant DNA comprising an insert coding for the variable regions of antibodies against HIV core protein p24 having the mentioned properties, methods of manufacture of such recombinant DNAs, and host cells transformed with such recombinant DNAs.
The antibodies are especially useful for the prevention of the progression of AIDS and for the treatment of AMS, but can also be used for the diagnosis of HIV infection in an immunoassay.

摘要翻译： 本发明涉及针对HIV核心蛋白p24的单克隆抗体和抗体衍生物，其识别在HIV感染的巨噬细胞的表面上表达的p24和/或杀死HIV感染的细胞。 本发明的单克隆抗体可以是由人恒定区和鼠可变区或高变区组成的鼠抗体或嵌合抗体。 本发明还包括制备这种抗体，分泌它们的杂交瘤或转染细胞系的方法和用于产生杂交瘤或转染细胞系的方法。 本发明还涉及包含编码具有所述性质的HIV核心蛋白p24抗体的可变区的插入片段的重组DNA，以及用这些重组DNA转化的宿主细胞的方法。 该抗体特别可用于预防AIDS的进展和用于治疗AMS，但也可用于免疫测定中HIV感染的诊断。

公开(公告)号：EP0502812B1

公开(公告)日：1996-08-14

申请号：EP92810056.9

申请日：1992-01-27

申请人： CIBA-GEIGY AG

发明人： Wels, Winfried Stephan, Dr. ,  Hynes, Nancy E., Dr. ,  Harwerth, Ina-Maria ,  Groner, Bernd, Dr. ,  Hardman, Norman, Dr. ,  Zwickl, Markus

IPC分类号： C12N15/62 ,  C12N1/21 ,  G01N33/574 ,  A61K39/395 ,  C07K16/30

CPC分类号： C07K16/32 ,  A61K38/00 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/55

公开(公告)号：EP0498767A2

公开(公告)日：1992-08-12

申请号：EP92810063.5

申请日：1992-01-28

申请人： CIBA-GEIGY AG

发明人： Hardman, Norman, Dr.

IPC分类号： C12N15/13 ,  C12P21/08 ,  C07K15/28 ,  A61K39/395 ,  G01N33/68

CPC分类号： C07K16/4258 ,  A61K38/00 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/02

摘要： The invention concerns chimeric antiidiotypic monoclonal antibodies consisting of human constant regions and murine variable regions of an antibody which recognizes antibodies directed against HLA class II antigens, and derivatives thereof which retain the specificity of the chimeric antibody from which they are derived. These chimeric antiidiotypic monoclonal antibodies and derivatives have immune-regulatory functions and can therefore be used for diagnostic and therapeutic purposes, such as the treatment of autoimmune diseases.

摘要翻译： 本发明涉及由人恒定区和识别针对HLA II类抗原的抗体的抗体的鼠可变区组成的嵌合抗独特型单克隆抗体及其衍生物，其保留了衍生自其的嵌合抗体的特异性。 这些嵌合抗独特型单克隆抗体和衍生物具有免疫调节功能，因此可用于诊断和治疗目的，例如治疗自身免疫性疾病。

公开(公告)号：EP0323806B1

公开(公告)日：1993-03-17

申请号：EP88810898.2

申请日：1988-12-28

申请人： CIBA-GEIGY AG

发明人： Hardman, Norman, Dr. ,  Gill, Laura Lee, Dr. ,  de Winter, Ronald F.J., Dr. ,  Wagner, Kathrin ,  Heusser, Christoph, Dr.

IPC分类号： C12N15/00 ,  A61K39/395 ,  C12P21/00 ,  C12N5/00 ,  G01N33/574

CPC分类号： C07K16/462 ,  A61K2039/505 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/33

公开(公告)号：EP0498767A3

公开(公告)日：1993-03-31

申请号：EP92810063.5

申请日：1992-01-28

申请人： CIBA-GEIGY AG

发明人： Hardman, Norman, Dr.

IPC分类号： C12N15/13 ,  C12P21/08 ,  C07K15/28 ,  A61K39/395 ,  G01N33/68

CPC分类号： C07K16/4258 ,  A61K38/00 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/02

摘要： The invention concerns chimeric antiidiotypic monoclonal antibodies consisting of human constant regions and murine variable regions of an antibody which recognizes antibodies directed against HLA class II antigens, and derivatives thereof which retain the specificity of the chimeric antibody from which they are derived. These chimeric antiidiotypic monoclonal antibodies and derivatives have immune-regulatory functions and can therefore be used for diagnostic and therapeutic purposes, such as the treatment of autoimmune diseases.

公开(公告)号：EP0502812A1

公开(公告)日：1992-09-09

申请号：EP92810056.9

申请日：1992-01-27

申请人： CIBA-GEIGY AG

发明人： Wels, Winfried Stephan, Dr. ,  Hynes, Nancy E., Dr. ,  Harwerth, Ina-Maria ,  Groner, Bernd, Dr. ,  Hardman, Norman, Dr. ,  Zwickl, Markus

IPC分类号： C12N15/62 ,  C12N1/21 ,  G01N33/574 ,  A61K39/395 ,  C07K16/30

CPC分类号： C07K16/32 ,  A61K38/00 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/55

摘要： The invention concerns recombinant antibodies directed to the extracellular domain of the human growth factor receptor c-erbB-2 comprising a light chain variable domain and a heavy chain variable domain of a monoclonal antibody, monoclonal antibodies directed to c-erbB-2 themselves, a method of manufacture of said recombinant antibodies and said monoclonal antibodies, hybridoma cells secreting said monoclonal antibodies, a method of manufacture of said hybridoma cells, DNA coding for the heavy chain variable domain, for the light chain variable domain and for the recombinant antibody, a method of manufacture of said DNA, hybrid vectors suitable for expression of said DNA, host cells transformed with said DNA, and the use of said recombinant antibodies and said monoclonal antibodies in the diagnosis and treatment of tumors.

摘要翻译： 本发明涉及针对人生长因子受体c-erbB-2的细胞外结构域的重组抗体，其包含轻链可变结构域和单克隆抗体的重链可变区，针对c-erbB-2本身的单克隆抗体， 所述重组抗体和所述单克隆抗体的制备方法，分泌所述单克隆抗体的杂交瘤细胞，所述杂交瘤细胞的制备方法，编码重链可变结构域的DNA，轻链可变结构域和重组抗体的制备方法 所述DNA的制备方法，适于表达所述DNA的杂交载体，用所述DNA转化的宿主细胞，以及所述重组抗体和所述单克隆抗体在肿瘤诊断和治疗中的用途。

公开(公告)号：EP0323806A1

公开(公告)日：1989-07-12

申请号：EP88810898.2

申请日：1988-12-28

申请人： CIBA-GEIGY AG

发明人： Hardman, Norman, Dr. ,  Gill, Laura Lee, Dr. ,  de Winter, Ronald F.J., Dr. ,  Wagner, Kathrin ,  Heusser, Christoph, Dr.

IPC分类号： C12N15/00 ,  A61K39/395 ,  C12P21/00 ,  C12N5/00 ,  G01N33/574

CPC分类号： C07K16/462 ,  A61K2039/505 ,  C07K16/3007 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/33

摘要： The invention relates to murine/human chimeric monoclonal antibodies with high specificity to and affinity for human carcinoembryonic antigen (CEA), derivatives thereof, processes for the preparation of these antibodies and their derivatives, DNAs coding for heavy and light chains of these antibodies, processes for the preparation of said DNAs, mammalian cell lines that produce and secrete the antibodies and processes for the preparation of said cell lines. The chimeric antibodies and their derivatives are used for clinical purposes in vitro and in vivo , especially for the diagnosis of cancer, for localization and in vivo imaging of tumors, for therapy, e.g. site-directed delivery of cytotoxins, and similar purposes. The invention also concerns test kits and pharmaceutical compositions containing said chimeric monoclonal antibodies and/or derivatives thereof.

摘要翻译： 本发明涉及对人癌胚抗原（CEA）具有高特异性和亲和力的鼠/人嵌合单克隆抗体，其衍生物，制备这些抗体及其衍生物的方法，编码这些抗体重链和轻链的DNA， 用于制备所述DNA，产生和分泌抗体的哺乳动物细胞系和用于制备所述细胞系的方法。 嵌合抗体及其衍生物用于体外和体内的临床目的，特别是用于癌症的诊断，肿瘤的定位和体内成像，用于治疗，例如。 细胞毒素的定点递送和类似目的。 本发明还涉及含有所述嵌合单克隆抗体和/或其衍生物的测试试剂盒和药物组合物。